PMID: 9657495Jul 10, 1998Paper

Nuclear multiparameter flow cytometric DNA analysis of human brain tumors: correlation of DNA content with tumor histology and clinical behavior

Surgical Neurology
M F ChiangY S Ho

Abstract

Using fresh surgical specimens of brain tumors to investigate cellular DNA content is uncommon. The aim of this study is to elucidate the relationship among cellular nuclear DNA content, tumor histology, and tumor clinical behavior of various tumors of the central nervous system. Multiparameter flow cytometry (FACStar, Becton-Dickinson, San Jose, CA) was randomly applied to analyze 40 fresh surgical specimens from brain tumors. Histologically, there were 19 cases of benign tumors and 21 cases of malignant tumors. DNA distribution profiles in the benign tumors revealed 15 cases of diploidy and four cases of aneuploidy, while the malignant tumors had eight cases of diploidy and 13 cases of aneuploidy (p < 0.01). The mean DNA index (DI) in benign tumors was 1.13 +/- 0.27 and in malignant tumors 1.43 +/- 0.48 (p < 0.05). The mean proliferative index (PI) in benign tumors was 10.27 +/- 5.46% and 18.44 +/- 8.98% in malignant tumors (p < 0.05). Clinically, 10 patients had postoperative recurrence. The PI in recurrent and nonrecurrent tumors was 17.83 +/- 11.13% and 13.20 +/- 7.19%, respectively (p < 0.05). The 1-year cumulative survival rate in benign tumors was 78.9%, and 57.1% in malignant tumors (p < 0.05). Malignant tumors had a s...Continue Reading

References

Jul 1, 1978·Journal of Neurosurgery·T HoshinoJ W Gray
Mar 15, 1978·Acta Neuropathologica·P FrederiksenP Bichel
Jun 13, 1975·Radiation and Environmental Biophysics·H BaischW A Linden
Apr 1, 1992·Cancer·J M KrosT H van der Kwast
Jan 1, 1990·Journal of Neuropathology and Experimental Neurology·P E McKeeverJ Varani
Jan 1, 1990·Oncology·R AssiettiP Gaetani
Jun 1, 1986·The Journal of Pathology·P Quirke, J E Dyson
Jan 1, 1989·Acta neurochirurgica·K KawamotoH Matsumura
Mar 1, 1989·Journal of Neurosurgery·M YasueK D Bauer
Jan 1, 1985·Acta neurochirurgica·D K LüdeckeW Schmidt
Dec 1, 1988·Journal of Neurosurgery·T HoshinoR L Davis
Dec 1, 1988·Journal of Neuro-oncology·K KawamotoA Ohyama
Jul 1, 1987·Journal of Neurosurgery·H H Schmidek
Dec 1, 1986·Journal of Neurosurgery·J W BookwalterM Kristofik
May 1, 1985·Journal of Neuropathology and Experimental Neurology·D J NishiguchiA J Yates
Jan 1, 1980·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K KawamotoL G Koss
Nov 1, 1980·Cytometry·J T ThornthwaiteW J Temple
Sep 1, 1984·Journal of Clinical Pathology·M L FriedlanderI W Taylor
Nov 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D W HedleyE A Musgrove
Apr 15, 1984·Cancer·M AnnikoJ Wersäll
Aug 1, 1980·Journal of Neurosurgery·S J Mørk, O D Laerum
Jul 1, 1993·Journal of Neuro-oncology·T NishizakiK Sasaki

❮ Previous
Next ❯

Citations

Jun 4, 2013·Journal of Neurosurgery·George VartholomatosSpyridon Voulgaris
Aug 19, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·John Ansell
Dec 21, 2002·Clinical Nuclear Medicine·Ilknur AkErkan Vardareli
Apr 16, 2002·Clinical Orthopaedics and Related Research·Henry J MankinAndrew E Rosenberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.