Nuclear organization and gene expression

Experimental Cell Research
D L Spector

Abstract

In actively transcribing cells, factors involved in pre-mRNA splicing localize in a speckled pattern at the fluorescence microscopic level. The speckled pattern corresponds to interchromatin granule clusters and perichromatin fibrils at the electron microscopic level. Based upon [3H]uridine incorporation studies transcription is thought to occur at the perichromatin fibrils and not within interchromatin granule clusters. We have shown that upon inhibition of RNA polymerase II transcription or pre-mRNA splicing, splicing factors redistribute and preferentially localize to interchromatin granule clusters, which become larger and more uniform in shape. Introduction of exogenous DNA templates into the cell nucleus results in a recruitment of splicing factors to the new sites of transcription. These data suggest that splicing factors are localized at storage and/or reassembly sites (interchromatin granule clusters) and are recruited to active sites of transcription (perichromatin fibrils). Furthermore, these data demonstrate that the speckled localization of pre-mRNA splicing factors is a reflection of the transcriptional and pre-mRNA splicing activities of the cell.

Citations

Sep 3, 1998·Molecular Biology of the Cell·M J HendzelD P Bazett-Jones
Sep 26, 2000·Human Molecular Genetics·N A LittleR C Davies
Oct 28, 2009·Genome Biology·Peter J Shepard, Klemens J Hertel
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Eilbracht, M S Schmidt-Zachmann
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kenji OhePaolo Sassone-Corsi
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·T KuesU Kubitscheck
Jan 29, 2000·The Journal of Cell Biology·J DostieN Sonenberg
Feb 16, 2010·Biochemical and Biophysical Research Communications·Ko EtoShin-ichi Abe
Dec 13, 2002·Applied Optics·Antonio Virgillo FaillaChristoph Cremer
Jan 22, 2005·Annals of the New York Academy of Sciences·Carlo Bertoni-FreddariManuela Malatesta
Oct 7, 2005·Microscopy Research and Technique·Manuela MalatestaCarlo Bertoni-Freddari
Jul 8, 2003·Microscopy Research and Technique·Manuela MalatestaGiancarlo Gazzanelli
Jan 31, 2007·Microscopy Research and Technique·Manuela MalatestaCristiano Bertolucci
Apr 10, 2008·Microscopy Research and Technique·Manuela MalatestaCarlo Zancanaro
Jan 18, 2005·Experimental Cell Research·Hiroshi MaitaSanae M M Iguchi-Ariga
Nov 25, 2004·Experimental Cell Research·Joanna M BridgerPeter Lichter
Jun 27, 2000·Biophysical Journal·H B SunH Yokota
Oct 19, 2000·Experimental Cell Research·F JauninS Fakan
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·D P Bazett-Jones, M J Hendzel
Jun 8, 2002·Biochemical and Biophysical Research Communications·Ping WangPin Ouyang
Jul 12, 2016·Biochemical and Biophysical Research Communications·Soo-Ho LeeHyun-Shik Lee
Sep 25, 1999·Journal of Cellular Physiology·N M MaraldiF A Manzoli
Jul 22, 1998·Journal of Cellular Biochemistry·B H Clubb, M Locke
Sep 16, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A EnkemannS L Berger

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