Nuclear protein phosphatase-1 regulates HIV-1 transcription.

The Journal of Biological Chemistry
Tatyana AmmosovaSergei Nekhai

Abstract

We recently reported that protein phosphatase 1 (PP1) dephosphorylates RNA polymerase II C-terminal repeats and regulates HIV-1 transcription in vitro. Here we provide evidence that PP1 is also required for Tat-induced HIV-1 transcription and for viral replication in cultured cells. Inhibition of PP1 by overexpression of nuclear inhibitor of PP1 (NIPP1) inhibited Tat-induced HIV-1 transcription in transient transfection assays. A mutant of NIPP1 that was defective in binding to PP1 did not have this effect. Also the co-expression of PP1 gamma reversed the inhibitory effect of NIPP1. Adeno-associated virus-mediated delivery of NIPP1 significantly reduced HIV-1 transcription induced by Tat-expressing adenovirus in CD4+ HeLa cells that contained an integrated HIV-1 promoter (HeLa MAGI cells). In addition, infection of HeLa MAGI cells with adeno-associated virus-NIPP1 prior to the infection with HIV-1 significantly reduced the level of HIV-1 replication. Our results indicate that PP1 might be a host cell factor that is required for HIV-1 viral transcription. Therefore, nuclear PP1 may represent a novel target for anti-HIV-1 therapeutics.

References

Jul 21, 1995·The Journal of Biological Chemistry·I JagielloM Bollen
Nov 21, 1998·The Journal of Biological Chemistry·N F MarshallM E Dahmus
Apr 10, 1999·Journal of Virology·W XiaoJ M Wilson
Mar 25, 2000·Molecular and Cellular Biology·D H Price
Feb 23, 2002·Trends in Cell Biology·Mathieu Bollen, Monique Beullens
Mar 23, 2002·The Journal of Biological Chemistry·Monique Beullens, Mathieu Bollen
Jul 2, 2002·Nature Medicine·Warner C Greene, B Matija Peterlin
Jul 10, 2002·The Journal of Biological Chemistry·An BoudrezMathieu Bollen
Aug 20, 2002·The Journal of Biological Chemistry·Kareem WashingtonSergei Nekhai

❮ Previous
Next ❯

Citations

Oct 14, 2010·Molecular and Cellular Biochemistry·Marina JerebtsovaSergei Nekhai
Dec 19, 2006·Oncogene·S NekhaiA L Gartel
Feb 9, 2005·Retrovirology·Nicolas EpieSergei Nekhai
Oct 5, 2006·Retrovirology·Nicolas EpieSergei Nekhai
Nov 7, 2006·Retrovirology·Tatyana AmmosovaSergei Nekhai
Dec 4, 2010·Retrovirology·Min XuSergei Nekhai
Nov 13, 2012·Retrovirology·Denitra BreuerSergei Nekhai
May 3, 2011·PloS One·Tatiana AmmosovaSergei Nekhai
Nov 21, 2012·PloS One·Mohamed Ali JarbouiVirginie W Gautier
Jul 25, 2009·Journal of Cellular Physiology·Sharroya CharlesSergei Nekhai
Feb 14, 2014·BioMed Research International·Sergei NekhaiDenitra Breuer
Aug 30, 2014·Critical Reviews in Microbiology·Shanshan LiXilan Yu
Jul 17, 2007·Virology·Zufan DebebeSergei Nekhai
Aug 9, 2005·Virology·Tatyana AmmosovaSergei Nekhai
Dec 6, 2016·Biology·Tatiana AmmosovaSergei Nekhai
Jan 1, 2012·Biology·Guillaume Mousseau, Susana Valente
Jul 14, 2006·Medicinal Research Reviews·Miguel StevensJan Balzarini
Oct 9, 2009·Immunological Investigations·Jessica L ReynoldsStanley A Schwartz
Sep 1, 2005·The Journal of Biological Chemistry·Tatyana AmmosovaSergei Nekhai
Aug 27, 2021·Toxins·Patrick Romano MonteiroAgenor Valadares Santos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.