PMID: 3769531Sep 1, 1986Paper

Nuclear pyknosis during lens fibre differentiation in epithelial explants

Current Eye Research
J W McAvoy, N A Richardson

Abstract

In our culture system, lens epithelial cells differentiate into fibres under the influence of fibre differentiation factor (FDF) from neural retinas. FDF stimulates DNA synthesis and cell migration within 24 hours. After 2 days we detect increased alpha-crystallin synthesis and, after 4 and 6 days, beta- and gamma-crystallin synthesis, respectively. In this study, a quantitative analysis of DNA levels using the DAPI method shows increases in DNA/explant up to 2 days of culture. This is followed by a substantial drop in DNA/explant by 10 days. The early rise in DNA levels corresponds with the period of cell division stimulated by FDF. The drop in DNA levels corresponds with a significant increase in the proportion of pyknotic nuclei in the explants. Electron microscopy shows pyknotic nuclei in differentiated fibre cells. Since nuclear pyknosis is a normal event during terminal fibre differentiation in vivo, this study confirms that our explant system faithfully reproduces events in fibre differentiation.

References

Nov 1, 1977·Analytical Biochemistry·J Kapuściński, B Skoczylas
Apr 1, 1986·Experimental Cell Research·M T Campbell, J W McAvoy
May 1, 1985·Australian and New Zealand Journal of Ophthalmology·J W McAvoyJ Walton
Jun 1, 1973·Experimental Eye Research·B D Srinivasan, T Iwamoto
Aug 1, 1984·Experimental Eye Research·J Walton, J McAvoy
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E LalandeR G Miller

❮ Previous
Next ❯

Citations

Apr 16, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Kevin A MarunoJohn W McAvoy
Feb 14, 1998·The Journal of Cell Biology·Y IshizakiM C Raff
Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael A Wride
Dec 1, 1996·Differentiation; Research in Biological Diversity·M A Wride
Nov 1, 1992·Experimental Eye Research·N A RichardsonC G Chamberlain
Dec 22, 2004·Progress in Retinal and Eye Research·T Stupp, S Thanos
Jun 9, 2006·Differentiation; Research in Biological Diversity·Qin YanDavid Wan-Cheng Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.