Nuclear Rad51 foci induced by DNA damage are distinct from Rad51 foci associated with B cell activation and recombination

Experimental Cell Research
M J Li, N Maizels

Abstract

Lipopolysaccharide (LPS) is a B cell mitogen which can stimulate murine primary B cells to proliferate and carry out immunoglobulin heavy chain class switch recombination. LPS can also function as an endotoxin, which may cause DNA damage and apoptosis in certain types of cells. We have previously reported that LPS-activated primary murine B cells contain nuclear foci that stain brightly with anti-Rad51 antibodies (Li et al. (1996) Proc. Natl. Acad. Sci. USA 93, 10222-10227). We have now analyzed Rad51 nuclear foci induced in both primary and immortalized B cells by treatment with the DNA damaging agent, methyl methanesulfonate (MMS). We have found that, in LPS-cultured primary B cells, MMS treatment increases the fraction of cells containing Rad51 foci and induces formation of a very high number of foci per cell. The foci induced by MMS treatment are small, punctate, and numerous; in contrast, the foci induced by LPS activation are large, brightly staining, and relatively few in number. In LPS-cultured primary B cells, Rad51 relocalizes during the cell cycle, and large, brightly staining nuclear foci are present in only restricted stages of the cell cycle. Rad51 foci similar to those present in LPS-activated primary B cells are...Continue Reading

Citations

May 29, 1998·Immunological Reviews·Q KongN Maizels
Aug 11, 2000·Molecular and Cellular Biology·M TakataS Takeda
Feb 28, 2002·Annual Review of Immunology·Tasuku HonjoMasamichi Muramatsu
Jan 1, 2006·The Arabidopsis Book·Hong Ma
Mar 21, 2001·EMBO Reports·Y Liu, N Maizels

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