Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells.

Chromosoma
Marilena CiciarelloPatrizia Lavia

Abstract

The GTPase Ran regulates nucleocytoplasmic transport in interphase and spindle organisation in mitosis via effectors of the importin beta superfamily. Ran-binding protein 1 (RanBP1) regulates guanine nucleotide turnover on Ran, as well as its interactions with effectors. Unlike other Ran network members that are steadily expressed, RanBP1 abundance is modulated during the mammalian cell cycle, peaking in mitosis and declining at mitotic exit. Here, we show that RanBP1 downregulation takes place in mid to late telophase, concomitant with the reformation of nuclei. Mild RanBP1 overexpression in murine cells causes RanBP1 to persist in late mitosis and hinders a set of events underlying the telophase to interphase transition, including chromatin decondensation, nuclear expansion and nuclear lamina reorganisation. Moreover, the reorganisation of nuclear pores fails associated with defective nuclear relocalisation of NLS cargoes. Co-expression of importin beta, together with RanBP1, however mitigates these defects. Thus, RanBP1 downregulation is required for nuclear reorganisation pathways operated by importin beta after mitosis.

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Citations

Dec 15, 2010·Journal of Cell Science·Maria GiubettiniGiulia Guarguaglini
Feb 15, 2012·The Journal of Cell Biology·Emanuele RoscioliPatrizia Lavia
Jan 23, 2016·Frontiers in Cell and Developmental Biology·Tommaso Cavazza, Isabelle Vernos
Nov 14, 2015·Molecular Biology of the Cell·Maiko FurutaTatsuo Fukagawa
Dec 3, 2014·Developmental Cell·Michael Shaofei ZhangMary Dasso
May 12, 2015·Biochimica Et Biophysica Acta·Henna V Kuusisto, David A Jans
Oct 29, 2014·Molecular and Cellular Biology·Javier Redondo-MuñozAna C Carrera
Jun 9, 2019·Journal of Neurodevelopmental Disorders·Zahra MotahariAnthony-Samuel LaMantia
Sep 20, 2020·International Journal of Molecular Sciences·Darina OcadlikovaAntonio Curti
Feb 19, 2021·Journal of Neuropathology and Experimental Neurology·Giorgia NapoletaniBianca De Filippis
Nov 12, 2018·Biochemical Pharmacology·Virginia CorreaniMaria d'Erme

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