Nuclear respiratory factor 2 senses changing cellular energy demands and its silencing down-regulates cytochrome oxidase and other target gene mRNAs

Gene
Sakkapol OngwijitwatM T Wong-Riley

Abstract

Cytochrome c oxidase (COX), the terminal enzyme of the electron transport chain, is a bigenomic enzyme with 13 subunits. The mechanism coordinating the transcription of these subunits is poorly understood. We investigated the role of nuclear respiratory factor-2 (NRF-2) in intragenomic regulation of nuclear COX genes. Vector-mediated short-hairpin RNA interference against NRF-2alpha reduced all 10 COX nuclear subunit mRNAs and mRNAs of other genes involved in mitochondrial function/biogenesis. NRF-2 binding site was necessary for the rat COX 4i1 promoter to down-regulate in response to decreased energy demands in primary neurons. Over-expression of NRF-2 protein prevented the down-regulation of transcriptional activity by TTX. Finally, NRF-2 binding sites in isolation were sufficient for modulating COX subunit 4i1 and 6A1 promoters' activity in response to decreased energy demand. These results indicate that NRF-2 is a vital part of a molecular mechanism that senses upstream energy signals and modulates COX transcriptional levels in mammalian cells.

References

Nov 1, 1991·Molecular and Cellular Biology·J V Virbasius, R C Scarpulla
Jan 1, 1988·Annual Review of Cell Biology·G Attardi, G Schatz
Aug 1, 1988·Molecular and Cellular Biology·R P Fisher, D A Clayton
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·M C Sorgato, O Moran
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 18, 2002·Nature Genetics·Maria FalkenbergClaes M Gustafsson
Oct 31, 2003·Molecular and Cellular Biology·Morten SunesenJean-Pierre Changeux
Dec 5, 2003·Nucleic Acids Research·Xiaowei Wang, Brian Seed
Mar 9, 2004·Genes & Development·Daniel P Kelly, Richard C Scarpulla
Jul 28, 2004·Gene·Sakkapol Ongwijitwat, Margaret T T Wong-Riley

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Citations

Mar 12, 2011·Journal of Physiology and Biochemistry·Akira WagatsumaShigeru Yamada
Jul 7, 2009·Annual Review of Physiology·M Benjamin Hock, Anastasia Kralli
Oct 4, 2012·BMC Neuroscience·J Annelies E PolmanNicole A Datson
Jul 9, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L R Jimenez-GutierrezA Muhlia-Almazan
Jul 24, 2012·Trends in Endocrinology and Metabolism : TEM·Richard C ScarpullaDaniel P Kelly
Jun 15, 2011·Molecular and Cellular Endocrinology·Joachim M Weitzel, K Alexander Iwen
Aug 11, 2010·Mitochondrion·Claudia BaltzerChristian Frei
Mar 25, 2008·Frontiers in Neuroendocrinology·David Crews
Jun 15, 2007·Neurobiology of Aging·Karen A BaskervilleMichael McKinney
Feb 16, 2007·Gene·José R BlesaJosé Hernández-Yago
Dec 17, 2008·Annals of the New York Academy of Sciences·Richard C Scarpulla
Jun 22, 2007·Glia·Christian GöritzFrank W Pfrieger
Sep 24, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K BremerC D Moyes
Nov 26, 2009·International Journal of Cancer. Journal International Du Cancer·Myoung Sook KimDavid Sidransky
Jul 23, 2013·Biochimica Et Biophysica Acta·Anusha PriyaMargaret T T Wong-Riley
Mar 1, 2014·Biochimica Et Biophysica Acta·Anusha PriyaMargaret T T Wong-Riley
Dec 22, 2010·Biochimica Et Biophysica Acta·Shilpa S Dhar, Margaret T T Wong-Riley
Jun 22, 2013·Autophagy·Jianhui ZhuCharleen T Chu
Sep 24, 2014·Biochimica Et Biophysica Acta·Anusha PriyaMargaret T T Wong-Riley
Aug 16, 2016·Molecular and Cellular Endocrinology·Shogo ShiratsukiHisataka Iwata
Oct 27, 2017·Archives of Physiology and Biochemistry·Abhijit Nirwane, Anuradha Majumdar
May 23, 2015·Circulation Research·Rick B VegaDaniel P Kelly
Dec 14, 2007·The Journal of Biological Chemistry·Shilpa S DharMargaret T T Wong-Riley
Apr 9, 2008·Physiological Reviews·Richard C Scarpulla

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