Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator.

The Journal of Cell Biology
Luciana Gómez AcuñaAlberto R Kornblihtt

Abstract

In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA-mediated posttranscriptional and also in transcriptional gene silencing. Here, we report a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells, AGO1 associates with transcriptional enhancers of estrogen receptor α (ERα) and that this association is up-regulated by treating the cells with estrogen (E2), displaying a positive correlation with the activation of these enhancers. Moreover, we show that AGO1 interacts with ERα and that this interaction is also increased by E2 treatment, but occurs in the absence of RNA. We show that AGO1 acts positively as a coactivator in estradiol-triggered transcription regulation by promoting ERα binding to its enhancers. Consistently, AGO1 depletion decreases long-range contacts between ERα enhancers and their target promoters. Our results point to a role of AGO1 in transcriptional regulation in human cells that is independent from small RNA binding.

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Methods Mentioned

BETA
immunoprecipitation
ChIP-seq
ChIP
PCR
confocal microscopy
coimmunoprecipitation
transfection
transfections
FCS
immunoprecipitations

Software Mentioned

FluoView Viewer
CellProfiler
HOMER
UCSC
deepTools
Bowtie
MACS2
SAMtools
BigWig

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