PMID: 11607225Oct 15, 1991Paper

Nuclear scaffolds and scaffold-attachment regions in higher plants

Proceedings of the National Academy of Sciences of the United States of America
G HallSteven Spiker

Abstract

DNA in the nuclei of eukaryotic organisms undergoes a hierarchy of folding to be packaged into interphase and metaphase chromosomes. The first level of packaging is the 11-nm nucleosome fiber, which is further coiled into a 30-nm fiber. Evidence from fungal and animal systems reveals the existence of higher order packaging consisting of loops of the 30-nm fibers attached to a proteinaceous nuclear scaffold by an interaction between the scaffold and specific DNA sequences called scaffold-attachment regions (SARs). Support for the ubiquitous nature of such higher order packaging of DNA is presented here by our work with plants. We have isolated scaffolds from tobacco nuclei using buffers containing lithium diiodosalicylate to remove histones and then using restriction enzymes to remove the DNA not closely associated with the scaffold. We have used Southern hybridization to show that the DNA remaining bound to the scaffolds after nuclease digestion includes SARs flanking three root-specific tobacco genes. This assay for SARs is termed the endogenous assay because it identifies genomic sequences as SARs by their endogenous association with the scaffold. Another assay, the exogenous assay, depends upon the ability of scaffolds to sp...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Nov 1, 1977·Cell·J R Paulson, U K Laemmli
Jun 1, 1990·Current Opinion in Cell Biology·S C Elgin
Dec 14, 1990·Science·L Manuelidis
May 1, 1988·Molecular & General Genetics : MGG·R C SykesA C Chinault
Mar 5, 1988·Journal of Molecular Biology·E IzaurraldeU K Laemmli
Sep 1, 1987·Molecular and Cellular Biochemistry·H C SmithA C Chinault
Jul 1, 1990·Plant Physiology·M A ConklingH M Goodman

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Citations

May 1, 1993·Journal of Cellular Biochemistry·T Boulikas
May 1, 1992·Plant Molecular Biology·P J Hooykaas, R A Schilperoort
Dec 1, 1996·Plant Molecular Biology·A M Chinn, L Comai
Aug 1, 1996·Chromosoma·P L StrisselH Swift
Nov 30, 2006·Journal of Applied Genetics·Marcin Filipecki, Stefan Malepszy
Jan 22, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hua XueCheng-Chao Zheng
Jul 5, 2006·Plant Cell Reports·Guangli SuoJianwu Dai
Dec 30, 2008·Plant Molecular Biology·Charles Q CaiJoseph F Petolino
May 11, 2010·Plant Molecular Biology·Joseph F PetolinoSean Russell
Oct 15, 1992·European Journal of Biochemistry·R S Jack, H Eggert
Jul 21, 2000·Genome Génome / Conseil National De Recherches Canada·C RampitschS Cloutier
Nov 13, 2012·PloS One·James R PetrieSurinder P Singh
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·G HansenM D Chilton
Apr 29, 1998·Proceedings of the National Academy of Sciences of the United States of America·G LiT C Hall
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·G Hansen, M D Chilton
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·G Hansen
Aug 8, 2006·The Plant Journal : for Cell and Molecular Biology·Tiehan ZhaoThomas Koprek
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Jan 30, 2015·Plant Biotechnology Journal·Gaurav KrishnaPramod W Ramteke
Sep 18, 1992·Cell·M E LudérusR van Driel
Apr 29, 2015·Journal of Biotechnology·Sandeep KumarManju Gupta
Sep 25, 2003·Journal of Cellular Biochemistry·Tomasz T CalikowskiIris Meier
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
Dec 15, 2006·Plant Biotechnology Journal·Apolonia H M Van der GeestWilliam M Ainley

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