Jul 2, 2014

Nuclear stability and transcriptional directionality separate functionally distinct RNA species

BioRxiv : the Preprint Server for Biology
Robin AnderssonAlbin Sandelin

Abstract

Mammalian genomes are pervasively transcribed, yielding a complex transcriptome with high variability in composition and cellular abundance. While recent efforts have identified thousands of new long non-coding (lnc) RNAs and demonstrated a complex transcriptional repertoire produced by protein-coding (pc) genes, limited progress has been made in distinguishing functional RNA from spurious transcription events. This is partly due to present RNA classification, which is typically based on technical rather than biochemical criteria. Here we devise a strategy to systematically categorize human RNAs by their sensitivity to the ribonucleolytic RNA exosome complex and by the nature of their transcription initiation. These measures are surprisingly effective at correctly classifying annotated transcripts, including lncRNAs of known function. The approach also identifies uncharacterized stable lncRNAs, hidden among a vast majority of unstable transcripts. The predictive power of the approach promises to streamline the functional analysis of known and novel RNAs.

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Mentioned in this Paper

Classification
Genome
Genes
RNA, Untranslated
Transcription, Genetic
Transcription Initiation
RNA, Long Untranslated
Species
Introns
Analysis

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