Nuclear suppressors define three factors that participate in both 5' and 3' end processing of mRNAs in Chlamydomonas chloroplasts

The Plant Journal : for Cell and Molecular Biology
Linda A RymarquisD B Stern

Abstract

Chloroplast RNA processing and degradation are orchestrated by nucleus-encoded factors. Although several transcript-specific factors have been identified, those involved in global RNA metabolism have mostly remained elusive. Using Chlamydomonas reinhardtii, we have identified three pleiotropic nuclear mutations, mcd3, mcd4 and mcd5, which cause quantitative variation between polycistronic transcripts and accumulation of transcripts with novel 3' ends. The mcd3, mcd4 and mcd5 mutants were initially isolated as photoautotrophic suppressors of the petD 5' mutants LS2 and LS6, which harbour four nucleotide linker-scanning mutations near the 5' end of the mature transcript. The LS mutants accumulate 1-3% of the wild-type (WT) petD mRNA level and no cytochrome b6/f complex subunit IV, which is the petD gene product and required for photosynthesis. Each suppressor restores approximately 15% of the WT petD mRNA and subunit IV levels. Genetic analysis showed mcd4 to be recessive, and suggested that MCD4 interacts with the petD mRNA stability factor MCD1. To assess the specificity of mcd3, mcd4 and mcd5, transcripts from 32 chloroplast genes were analysed by RNA filter hybridizations. mcd3 and mcd4 displayed aberrant transcript patterns ...Continue Reading

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May 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Vanessa KhemiciAgamemnon J Carpousis

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Citations

Jun 3, 2009·Current Genetics·Simon GeimerJuraj Krajcovic
Jun 18, 2010·Biotechnology Letters·Elizabeth SpechtStephen Mayfield
Nov 13, 2007·The Plant Journal : for Cell and Molecular Biology·Michel Goldschmidt-ClermontJean-David Rochaix
Jun 27, 2012·Molecular Biology Reports·Amir Ata SaeiAbolfazl Barzegari

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