Abstract
TLR signaling is associated with the transcription of various proinflammatory cytokines, including TNF-α, IL-6, and IL-1β. After transcription, the mRNA of these proinflammatory cytokines needs to be tightly controlled at the posttranscriptional level to achieve an optimal expression. However, the precise mechanism of posttranscriptional regulation is not fully understood. In the current study, we found the expression of heterogeneous nuclear ribonucleoprotein U (hnRNP U), also termed scaffold attachment factor A, was greatly induced by TLR stimulation in macrophages. Knockdown of hnRNP U expression greatly attenuated TLR-induced expression of TNF-α, IL-6, and IL-1β, but not IL-12, whereas hnRNP U overexpression greatly increased TLR-induced expression of TNF-α, IL-6, and IL-1β. Furthermore, hnRNP U knockdown accelerated the turnover and decreased the t(1/2) of TNF-α, IL-6, and IL-1β mRNA. RNA immunoprecipitation demonstrated that hnRNP U bound to the mRNA of these proinflammatory cytokines through the RGG motif. Importantly, we showed that TLR stimulation provided a stimulus for hnRNP U nuclear to cytoplasmic translocation. Therefore, we propose that hnRNP U induced by TLR signaling binds to the mRNA of a subset of proinflamma...Continue Reading
References
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Nov 14, 1997·The Journal of Biological Chemistry·M EggertR Renkawitz
Feb 21, 1998·The EMBO Journal·F GöhringF O Fackelmayer
Feb 19, 2002·Genes & Development·Matti DavisYinon Ben-Neriah
Mar 23, 2002·Molecular and Cellular Biology·Joost H A MartensAlt Zantema
Aug 29, 2003·Molecular and Cellular Biology·Bedabrata SarkarRobert J Schneider
Apr 21, 2004·The Journal of Biological Chemistry·Michael HowellSharon L Milgram
Jun 2, 2005·Nature Reviews. Immunology·Foo Y LiewLuke A J O'Neill
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chengjiang GaoPaul C Kuo
Jul 19, 2005·Transgenic Research·Michael J Roshon, H Earl Ruley
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Feb 25, 2006·The EMBO Journal·Jae-Sung WooByung-Ha Oh
Mar 23, 2006·Annual Review of Immunology·Bruce BeutlerKasper Hoebe
May 10, 2006·Advances in Immunology·Georg Stoecklin, Paul Anderson
Aug 23, 2006·Oncogene·L SpraggonC Miles
Sep 7, 2006·Molecular and Cellular Biology·Serge PaschoudLukas C Kühn
Nov 7, 2006·Genes & Development·Jin-Yu LuRobert J Schneider
Mar 20, 2008·Nature Immunology·Paul Anderson
Aug 20, 2008·Molecular and Cellular Biology·Ales ObrdlikPiergiorgio Percipalle
Apr 9, 2009·Biochemical and Biophysical Research Communications·Fredrik M Berglund, Paul R Clarke
Jul 21, 2009·The Journal of Biological Chemistry·Maya Ameyar-ZazouaAnnick Harel-Bellan
Oct 22, 2009·Cell Cycle·Sébastien BrittonPatrick Calsou
Feb 13, 2010·Development·Dieter PullirschAnton Wutz
Apr 21, 2010·Nature Immunology·Taro Kawai, Shizuo Akira
Apr 30, 2010·The Journal of Biological Chemistry·Wei ZhaoChengjiang Gao
May 25, 2010·Cellular and Molecular Life Sciences : CMLS·Khalid S A Khabar
Jun 18, 2010·The Journal of Biological Chemistry·Shinji KawanoKen Tsutsui
Aug 28, 2010·The Biochemical Journal·Siew Ping HanRoss Smith
Aug 28, 2010·Molecular Cell·Yoshihiro TsuchiyaHideaki Kamata
Sep 14, 2010·Developmental Cell·Yuko HasegawaShinichi Nakagawa
Citations
Jan 10, 2014·Pharmacogenetics and Genomics·Michael HarrisJohn Deeken
Jan 4, 2013·PloS One·Shuzhen GuoEng H Lo
May 10, 2013·PloS One·Rong GuoXinmin Liu
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Samiran MondalTasuku Honjo
Sep 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hironori NishitsujiKunitada Shimotohno
Oct 7, 2016·Frontiers in Cellular and Infection Microbiology·Xiaopeng ZhangXiancai Rao
Oct 11, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yajing LuRui Zhou
Jun 29, 2017·Journal of Cellular Biochemistry·Yuan GaoYun Qi
Jan 20, 2018·Cellular and Molecular Life Sciences : CMLS·Wenwen WangJianni Qi
Feb 27, 2014·Biotechnology & Genetic Engineering Reviews·Takashi Mino, Osamu Takeuchi
Apr 3, 2020·Cancer Medicine·Jianyuan PanYu Wang
Apr 18, 2020·PloS One·Masato YugamiMasato Yano
Oct 25, 2017·Scientific Reports·Tidi HassanCatherine M Greene
Feb 14, 2014·The Journal of Biological Chemistry·Xueling LiAndrzej Kudlicki
Sep 28, 2017·International Journal of Molecular Medicine·Hongjun BianJianni Qi
Nov 7, 2019·Cell Reports·Kelsi O WestRobert O Watson
Jul 27, 2021·Frontiers in Immunology·Allison R WagnerKristin L Patrick