Nuclear topography of beta-like globin gene cluster in IL-3-stimulated human leukemic K-562 cells

Blood Cells, Molecules & Diseases
Gabriela GaliováStanislav Kozubek

Abstract

The beta-like globin genes, Ggamma, Agamma, delta and beta, forming specific clusters on chromosome 11, are transcriptionally regulated by the locus control region (LCR). The members of beta-like globin gene cluster (11p15.4) are variously switched during ontogenetic dependent erythropoiesis; however, changes of globin gene expression can be also observed during erythroid differentiation of bone marrow cells. In our experiments, interleukin-3 (IL-3)-stimulated human leukemic K-562 cells were used as a model system in which nuclear organization and expression of the beta-like globin gene cluster was investigated. In addition, the influence of IL-3 on the arrangement of chromosome 11 territory was analyzed. We observed that the beta-globin gene is not expressed in progenitor (nondifferentiated) K-562 cells, but is, however, activated after IL-3 stimulation of the K-562 population. A similar nuclear location of beta-like globin gene clusters was found in both control and IL-3-treated cells, which indicates that changes in cluster gene expression are accompanied by conserved nuclear topography of the gene cluster studied. On the other hand, the studied type of cell differentiation was characterized by relocation of chromosome 11 an...Continue Reading

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Citations

Mar 6, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Eva BártováStanislav Kozubek
Nov 24, 2004·BMC Cell Biology·Sonja StadlerSteffen Dietzel
Apr 29, 2010·Cell Structure and Function·Andrea Harnicarová HorákováStanislav Kozubek
Feb 19, 2010·Folia Histochemica Et Cytobiologica·Bartosz MikołajczakMaciej Kurpisz
Jun 7, 2008·Leukemia Research·Jana KrejcíEva Bártová
Oct 6, 2015·Annual Review of Genetics·Huy Q Nguyen, Giovanni Bosco
May 18, 2006·Biology of the Cell·Eva Bártová, Stanislav Kozubek
Aug 6, 2011·Nucleus·Megan J PuckelwartzElizabeth M McNally
Sep 20, 2018·Journal of Assisted Reproduction and Genetics·Zaida SarrateJoan Blanco
Dec 2, 2006·Seminars in Cancer Biology·Karen J MeaburnEvi Soutoglou
Feb 14, 2008·Journal of Cell Science·Katie A FinchDarren K Griffin

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