PMID: 9529092Apr 7, 1998Paper

Nuclear translocation of NF-kappaB in lipopolysaccharide-treated macrophages fails to correspond to endotoxicity: evidence suggesting a requirement for a gamma interferon-like signal

Infection and Immunity
L C DenlingerP J Bertics

Abstract

Elucidation of a signal transduction pathway essential to lipopolysaccharide (LPS)-induced macrophage activation has the capacity to provide new targets for the treatment of septic shock. In this regard, activation of the transcription factor NF-kappaB is commonly thought to be critical to LPS-stimulated macrophage inflammatory mediator production, although certain immunological, genetic, and molecular evidence suggests that other factors are involved. To address this issue, we hypothesized that the degree of LPS-induced NF-kappaB mobilization should correlate with the murine endotoxicity of the species of LPS used for in vitro study. Therefore, using D-galactosamine-sensitized mice, we assessed the lethal potencies of eight LPS preparations from Escherichia, Salmonella, Klebsiella, Bacteroides, Pseudomonas, Neisseria, and Rhodobacter species as well as that of the endotoxin substructure lipid X. The lethal potencies of these LPS preparations varied by > 160-fold. Treatment of RAW 264.7 cells with the same LPS preparations induced levels of tumor necrosis factor alpha (TNF-alpha) and NO production that correlated with the LPS 50% lethal dose. The combined analysis of the levels of these two mediators produced in response to LPS...Continue Reading

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Citations

Dec 6, 2000·Immunopharmacology and Immunotoxicology·S PolletA Favier
Apr 14, 2018·Frontiers in Immunology·Alan Y HsuQing Deng
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabine TötemeyerClare Bryant
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L C DenlingerP J Bertics
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul G ThomasDonald A Harn
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L A CramerM J Klemsz
Apr 12, 2008·Purinergic Signalling·Alma N GuerraPaul J Bertics
Feb 27, 2007·Veterinary Immunology and Immunopathology·Clare E BryantB A Farnfield
Mar 20, 1999·Immunobiology·D C NeujahrD S Pisetsky

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