Nuclear transport of granzyme B (fragmentin-2). Dependence of perforin in vivo and cytosolic factors in vitro.

The Journal of Biological Chemistry
D A JansJ A Trapani

Abstract

Cytotoxic T and natural killer cells are able to kill their target cells through synergistic action of the pore-forming protein perforin and the serine protease granzyme B, resulting in very distinctive nuclear changes typical of apoptosis. Whereas perforin acts at the membrane, granzyme B appears to be both capable of entering the cytoplasm of target cells and accumulating in isolated nuclei. In this study we examine nuclear transport of fluoresceinated granzyme B both in vivo in intact cells in the presence of perforin and in vitro in semi-permeabilized cells using confocal laser scanning microscopy. Granzyme B alone was observed to enter the cytoplasm of intact cells but did not accumulate in nuclei. In the presence of sublytic concentrations of perforin, however, it accumulated strongly in intact cell nuclei to levels maximally about 1.5 times those in the cytoplasm after about 2.5 h. In vitro nuclear transport assays showed maximal levels of nuclear and nucleolar accumulation of granzyme B of about 2.5- and 3-fold those in the cytoplasm. In contrast to signal-dependent nuclear accumulation of SV40 large tumor antigen (T-Ag) fusion proteins in vitro, nuclear/nucleolar import of granzyme B was independent of ATP and not inhi...Continue Reading

References

Nov 1, 1992·Journal of Leukocyte Biology·J R OrtaldoK Okumura
Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J CleverH Kasamatsu
Feb 1, 1992·The Journal of Experimental Medicine·L ShiA H Greenberg
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M F Crouch
Jan 1, 1991·Journal of Virology·K Martin, A Helenius
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J L MeinkothJ R Feramisco
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Oct 1, 1989·The Journal of Experimental Medicine·R C DukeJ D Young
Nov 1, 1987·Molecular and Cellular Biology·R B MorelandL M Hereford
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A TrapaniB Dupont
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C G LobeR C Bleackley
Aug 29, 1986·Cell·J D Young, Z A Cohn
Jan 1, 1985·Annual Review of Immunology·P A Henkart
Nov 1, 1995·The Biochemical Journal·D A Jans
May 1, 1994·Trends in Biochemical Sciences·M S Moore, G Blobel
Jun 1, 1995·Current Opinion in Cell Biology·W C Earnshaw
Apr 1, 1995·Immunology Today·M J Smyth, J A Trapani
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A Jans
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·P K HeplerP C John
Sep 15, 1993·Biochemical and Biophysical Research Communications·J A TrapaniM J Smyth
Apr 26, 1996·The Journal of Biological Chemistry·M J PinkoskiR C Bleackley
Jul 1, 1996·Physiological Reviews·D A Jans, S Hübner
Nov 1, 1991·Trends in Cell Biology·M L SchmitzP A Baeuerle

❮ Previous
Next ❯

Citations

May 26, 2001·Biochemical and Biophysical Research Communications·R LixinD A Jans
Feb 7, 2002·Biochemical and Biophysical Research Communications·Yoshio Yaoita
Apr 16, 1998·Springer Seminars in Immunopathology·J A TrapaniV R Sutton
Aug 4, 1999·Immunology Today·J A TrapaniM J Smyth
Oct 31, 1998·Current Opinion in Immunology·S ShrestaT J Ley
Nov 5, 2013·Cell Death and Differentiation·S A H de PootN Bovenschen
Jan 5, 1999·The Journal of Experimental Medicine·G MacDonaldA H Greenberg
Feb 28, 2002·Annual Review of Immunology·John H Russell, Timothy J Ley
Feb 29, 2008·Annual Review of Immunology·Dipanjan Chowdhury, Judy Lieberman
Apr 29, 1998·Annual Review of Physiology·V J Kidd
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·W LiH M Kagan
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S C PillerD A Jans
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C T Pham, T J Ley
Jan 23, 2003·The Journal of Cell Biology·Joseph A TrapaniKylie A Browne
Apr 5, 2013·Immunological Reviews·Marshall P Thomas, Judy Lieberman
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·G Bauer
May 16, 2006·FEMS Yeast Research·Kaleb J MarrChristopher H Mody
Dec 9, 2016·Cell Structure and Function·Mika HoriguchiYusuke Ohba
Jan 27, 1998·The Journal of Biological Chemistry·A EfthymiadisD A Jans
Sep 20, 2000·Leukemia·D E Johnson
Mar 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Richard N Re
Jun 1, 2010·Journal of Cellular Biochemistry·Bárbara Lara-ChacónBulmaro Cisneros
Jun 12, 2010·Immunological Reviews·Desiree A AnthonyMark J Smyth
May 27, 2003·Nature Reviews. Immunology·Judy Lieberman
Mar 14, 2000·Cell Death and Differentiation·M J PinkoskiR C Bleackley
Dec 31, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alex J FulcherDavid A Jans

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis