Nuclear YAP localization as a key regulator of podocyte function.

Cell Death & Disease
J BonseB Vollenbröker

Abstract

Podocytes are crucial for the establishment of the blood-urine filtration barrier in the glomeruli of the kidney. These cells are mainly affected during glomerulopathies causing proteinuria and kidney function impairment. Ongoing podocyte injury leads to podocyte loss, finally followed by end-stage kidney disease. Podocytes display a predominant nuclear localization of YAP (Yes-associated protein), one effector protein of the Hippo pathway, which regulates the balance between proliferation, differentiation, and apoptosis in cells. Nuclear active YAP seems to be critical for podocyte survival in vivo and in vitro. We can show here that different treatments leading to sequestration of YAP into the cytoplasm in podocytes, like decreased rigidity of the substrate, incubation with dasatinib, or overexpression of Hippo pathway members result in the induction of apoptosis. A RNA sequencing analysis of large tumor suppressor kinase 2 (LATS2) overexpressing podocytes confirmed a significant upregulation of apoptotic genes. The downregulation of Hippo pathway components suggests a feedback mechanism in podocytes. Noteworthy was the regulation of genes involved in cell-cell junction, the composition of the extracellular space, and cell mi...Continue Reading

References

Oct 28, 2005·Journal of the American Society of Nephrology : JASN·Astrid WeinsMartin R Pollak
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 4, 2008·Journal of the American Society of Nephrology : JASN·Kerstin DuningHermann Pavenstädt
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Dec 22, 2009·Nature Genetics·Elizabeth J BrownMartin R Pollak
Sep 18, 2010·The American Journal of Pathology·Jeffrey B HodginVivette D D'Agati
Jan 14, 2011·Current Drug Targets·Antonio GnoniVito Lorusso
Feb 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristen L MeerbreyStephen J Elledge
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Jun 11, 2011·Contributions to Nephrology·Behzad NajafianAgnes B Fogo
Nov 8, 2011·Annual Review of Physiology·Anna Greka, Peter Mundel
Apr 21, 2012·Journal of the American Society of Nephrology : JASN·Rizaldy P ScottTony Pawson
Apr 2, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Eric WallaceHuma Fatima
May 15, 2013·The Journal of Biological Chemistry·Kirk N CampbellPeter Mundel
Aug 21, 2013·Genome Research·Wenjun JuMatthias Kretzler
Dec 7, 2013·Biochemical and Biophysical Research Communications·Hai Fang, Julian Gough
Dec 7, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rebecca L RuebnerBernard S Kaplan
Mar 1, 2014·Molecular & Cellular Proteomics : MCP·Ulf SchulzeThomas Weide
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 29, 2015·The Journal of Cell Biology·Rizaldy P Scott, Susan E Quaggin
May 28, 2015·Journal of the American Society of Nephrology : JASN·Monica SchwartzmanKirk N Campbell
Sep 1, 2015·Cell Research·Xiaoming DaiBin Zhao
Jan 6, 2016·Genes & Development·Zhipeng MengKun-Liang Guan
Feb 24, 2016·Cellular Signalling·Lily HoaAlexander Hergovich
Feb 6, 2017·Journal of the American Society of Nephrology : JASN·Thomas WeideHermann Pavenstädt
Apr 13, 2017·Science Signaling·Markus M RinschenBernhard Schermer
Jul 9, 2017·Pflügers Archiv : European journal of physiology·Karlhans EndlichNicole Endlich
Jul 12, 2017·Journal of the American Society of Nephrology : JASN·Daniel GranadoThomas Weide

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Citations

Mar 20, 2020·Journal of the American Society of Nephrology : JASN·Jun MatsudaTomoko Takano
Nov 19, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Qiyuan ZhuangHuijuan Wu
Mar 11, 2021·Journal of the American Society of Nephrology : JASN·Annika Möller-KeruttThomas Weide
Apr 15, 2021·Canadian Journal of Physiology and Pharmacology·Junjie ZhangMing-Sheng Zhou

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Methods Mentioned

BETA
Assay
PCR
fluorescence-activated cell sorting
biopsies

Software Mentioned

GraphPad Prism
Nephroseq
BD [UNK] Pro
ImageJ
pINDUCER
Cufflinks
supraHex
Trimmomatic
CummeRbund
TopHat

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