Nuclease resistance of telomere-like oligonucleotides monitored in live cells by fluorescence anisotropy imaging

Analytical Chemistry
Zehui Charles CaoWeihong Tan

Abstract

Telomeres carry important biological functions such as the protection of chromosomes. In this paper, we have developed a fluorescence anisotropy imaging system for monitoring DNA digestion inside live cells. The nuclease-resistant capability of telomere-like ssDNAs in nuclei of human breast cancer cells is studied. We found that those oligonucleotides were clearly more stable than regular DNA sequences during the time course of the experiments. We conclude that the G-quadruplex structure of the telomere-like ssDNA makes it inherently more stable in intracellular environments than non-G-quadruplex structures. This will help us understand why the G-quadruplex forming telomere sequences were adopted by almost all eukaryotic cells to protect the ends of chromosomes. This is the first time such a phenomenon was observed in live cells. Our fluorescence anisotropy imaging provides an efficient way to directly monitor DNA digestion in any region of live cells in real time, providing insights into many important and related intracellular processes.

References

May 31, 1990·Nature·C B HarleyC W Greider
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L A KlobutcherD M Prescott
Dec 8, 1995·Science·V A Zakian
Mar 8, 1996·The Journal of Biological Chemistry·J S BishopN Chaudhary
May 25, 1999·Cell·C W Greider
Apr 13, 2001·Neuron·K HaasH T Cline
May 16, 2003·Optics Letters·Chad E BigelowThomas H Foster
Mar 24, 2004·Optics Letters·Klaus SuhlingPaul M W French
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Titia de Lange

❮ Previous
Next ❯

Citations

Jan 1, 2009·Analytical Chemistry·Joseph A PhillipsWeihong Tan
Dec 26, 2007·Nucleic Acids Research·Jun TangZheng Tan
Jan 30, 2007·Breast Cancer Research : BCR·Mina YaarBarbara A Gilchrest
Apr 18, 2014·Nature Reviews. Cancer·James R W ConwayPaul Timpson
Jul 24, 2010·Journal of Oncology·Christina ColemanDavid A Goukassian
Feb 10, 2016·Nucleic Acid Therapeutics·Olof GissbergC I Edvard Smith
Jan 19, 2016·Breast Cancer Research and Treatment·Johanna M TuomelaKatri S Selander
May 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alexander C HoepkerGerard Marriott
Dec 5, 2009·Biosensors & Bioelectronics·Tao BingDihua Shangguan
Mar 10, 2009·Current Opinion in Biotechnology·James A LevittKlaus Suhling
May 21, 2009·Experimental and Molecular Pathology·Paula J BatesJohn O Trent
Nov 13, 2008·International Journal of Cancer. Journal International Du Cancer·Harold O LongeGerald V Denis
Oct 23, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Joonas H KauppilaKatri S Selander
Dec 9, 2014·Artificial DNA, PNA & XNA·Natalia A KolganovaEdward N Timofeev
Sep 24, 2015·PloS One·Jossimara PolettiniRamkumar Menon
May 30, 2007·Molecular BioSystems·Frederic FestyKlaus Suhling
Feb 15, 2020·Cell Biochemistry and Function·Wen-Fang YuanJiang-Feng Wu
Mar 31, 2021·Nucleic Acids Research·Ernest WangJiou Wang
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner
Oct 28, 2014·Biochemistry·Petra TóthováViktor Víglaský
Jun 25, 2008·Analytica Chimica Acta·Joseph A PhillipsWeihong Tan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chembiochem : a European Journal of Chemical Biology
Dihua ShangguanWeihong Tan
Proceedings of the National Academy of Sciences of the United States of America
R F MacayaJ Feigon
The Journal of Biological Chemistry
Krzysztof SobczakWlodzimierz J Krzyzosiak
© 2021 Meta ULC. All rights reserved