Nuclease sensitive element binding protein 1 associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation

Journal of Cellular Physiology
Qichang ShenP E Newburger

Abstract

Biosynthesis of selenium-containing proteins requires insertion of the unusual amino acid selenocysteine by alternative translation of a UGA codon, which ordinarily serves as a stop codon. In eukaryotes, selenoprotein translation depends upon one or more selenocysteine insertion sequence (SECIS) elements located in the 3'-untranslated region of the mRNA, as well as several SECIS-binding proteins. Our laboratory has previously identified nuclease sensitive element binding protein 1 (NSEP1) as another SECIS-binding protein, but evidence has been presented both for and against its role in SECIS binding in vivo and in selenoprotein translation. Our current studies sought to resolve this controversy, first by investigating whether NSEP1 interacts closely with SECIS elements within intact cells. After reversible in vivo cross-linking and ribonucleoprotein immunoprecipitation, mRNAs encoding two glutathione peroxidase family members co-precipitated with NSEP1 in both human and rat cell lines. Co-immunoprecipitation of an epitope-tagged GPX1 construct depended upon an intact SECIS element in its 3'-untranslated region. To test the functional importance of this interaction on selenoprotein translation, we used small inhibitory RNAs to r...Continue Reading

References

Dec 1, 1991·Trends in Biochemical Sciences·A BöckC Baron
May 1, 1991·Archives of Biochemistry and Biophysics·R S EsworthyJ H Doroshow
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F ZinoniA Böck
Jan 1, 1990·Annual Review of Biochemistry·T C Stadtman
Jan 1, 1990·Annual Review of Nutrition·R A Sunde
Jul 1, 1987·Journal of Inorganic Biochemistry·M L Hu, A L Tappel
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K DidierB D Schwartz
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·S C VendelandP D Whanger
Oct 1, 1994·The Journal of Nutrition·R F Burk, K E Hill
Jan 1, 1993·Annual Review of Nutrition·R F Burk, K E Hill
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C BaronA Böck
Jan 1, 1995·Annual Review of Nutrition·P R Larsen, M J Berry
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·P E Shockett, D G Schatz
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·V N GladyshevT C Stadtman
Jan 1, 1996·Annual Review of Biochemistry·T C Stadtman
Apr 13, 2000·Journal of Cellular Biochemistry·R WuP E Newburger
Nov 15, 2000·Biological Chemistry·J KöhrlL Flohé
Mar 27, 2001·EMBO Reports·R M TujebajevaM J Berry
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E E CapowskiJ S Malter
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Somashe NiranjanakumariMariano A Garcia-Blanco
Dec 18, 2002·The EMBO Journal·Martin Thanbichler, August Böck
Jan 14, 2003·Annual Review of Nutrition·Donna M Driscoll, Paul R Copeland
Jun 20, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kimitoshi KohnoMichihiko Kuwano
Jul 9, 2004·Nature·Carl D Novina, Phillip A Sharp
Sep 17, 2004·Nature·Craig C Mello, Darryl Conte

❮ Previous
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Citations

Feb 17, 2009·Molecular and Cellular Biochemistry·Filomena G OttavianoJoseph Loscalzo
Nov 20, 2010·Antioxidants & Redox Signaling·Edith LubosDiane E Handy
Dec 27, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Filomena G OttavianoJoseph Loscalzo
Mar 17, 2009·Biochimica Et Biophysica Acta·Christine AllmangAlain Krol
Jun 2, 2006·Biochimie·C Allmang, A Krol
Jul 25, 2009·The Biochemical Journal·Frederick P BellingerMarla J Berry
Dec 4, 2012·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Matilde Maiorino
Jan 25, 2017·Nucleic Acids Research·Anne-Sophie Gribling-BurrerChristine Allmang
Dec 18, 2013·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Megan RechMark Mlynarek
Nov 28, 2013·Antioxidants & Redox Signaling·Sandra SeeherUlrich Schweizer
Aug 1, 2013·Biomolecular Concepts·Jonathan N Gonzalez-FloresPaul R Copeland
Mar 8, 2020·Inflammopharmacology·Sneha Hariharan, Selvakumar Dharmaraj

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