Nuclei of oocytes derived from mouse parthenogenetic embryos are competent to support development to term

Biology of Reproduction
Katsutoshi NiwaTomohiro Kono

Abstract

Mouse parthenotes result in embryonic death before 10 days of gestation, but parthenogenetic embryos (ng/fg PE) that contain haploid sets of genomes from nongrowing (ng) oocytes derived from newborn fetuses and fully grown (fg) oocytes derived from adults can develop into 13.5-day-old fetuses. This prolonged development is due to a lack of genomic imprinting in ng oocytes. Here, we show maternal genomes of oocytes derived from ng/fg PE are competent to support normal development. After 28 days of culture, the ovaries from ng/fg PE grew as well as the controls, forming vesicular follicles with follicular antrums. The oocytes collected from the developed follicles were the same size as those of the controls. To determine whether maternal primary imprinting had been established in the oocytes derived from ng/fg PE, we examined the DNA methylation status in differentially methylated regions of three imprinted genes, Igf2r, Lit1, and H19. The results showed that maternal-specific modifications were imposed in the oocytes derived from ng/fg PE. Further, to assess nuclear competence to support development, we constructed matured oocytes containing a haploid genome derived from ng/fg PE oocytes by serial nuclear transfer. After in vitr...Continue Reading

References

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Citations

Feb 16, 2008·Nature Protocols·Manabu KawaharaTomohiro Kono
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Wei ShenHongkui Deng
May 10, 2016·Theriogenology·Lessly P Sepulveda-RinconNathalie Beaujean
May 26, 2006·Biology of Reproduction·Wei ShenHongkui Deng

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Methods Mentioned

BETA
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