PMID: 8955875Nov 1, 1996Paper

Nucleic acid-8-methoxypsoralen crosslinks bind monoclonal anti-Z-DNA antibody

Biochemistry and Molecular Biology International
Z Arif, R Ali

Abstract

Native calf thymus DNA and poly(dA-dT).poly(dA-dT) were photo-adducted with 8-methoxypsoralen and characterized by thermal denaturation (Tm) and hydroxyapatite column chromatography. The data demonstrated the formation of interstrand photo-crosslinks. It has been shown by competition ELISA and band shift assays that crosslinked species of DNA-8-MOP and poly(dA-dT)-8-MOP photoadducts recognize previously defined monoclonal anti-Z-DNA antibody (Z22). The results indicate the possible presence of Z- or Z-like epitopes on nucleic acid-8-MOP crosslinks as Z22 antibody does not recognize other nucleic acid conformations. These studies also point out that conformational changes in DNA arising from the photo-addition could induce antibodies to DNA or could cause autoimmune disease.

Citations

Feb 13, 2014·International Journal of Biological Macromolecules·Badar Ul IslamAsif Ali

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