PMID: 7518919Jul 11, 1994Paper

Nucleic acid binding and intracellular localization of unr, a protein with five cold shock domains

Nucleic Acids Research
H Jacquemin-SablonF Dautry

Abstract

The unr gene was identified as a transcription unit located immediately upstream of N-ras in the genome of several mammalian species. While this genetic organization could be important for the transcriptional regulation of unr and N-ras, the function of the protein product of unr is unknown. unr is ubiquitously expressed and codes for an 85 kDa protein which is not closely related to previously characterized proteins. Nevertheless, a search for protein motifs has indicated the presence of five 'cold shock domains' within unr, a motif present in procaryotic cold shock proteins and in the vertebrate Y box factors. As these proteins have been reported to interact with nucleic acids, we investigated whether unr could bind to some classes of nucleic acids. We report here that unr has a high affinity for single-stranded DNA or RNA and a low affinity for double-stranded nucleic acids. Its low affinity for double-stranded DNA clearly distinguishes unr from the Y box factors. The binding of unr to RNA does not appear to depend upon extended sequence motifs but requires some level of sequence complexity as unr has only a low affinity for most simple polymers including polyA stretches. unr is also characterized by its low affinity for dou...Continue Reading

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Citations

Jun 23, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Sommerville
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Graumann, M A Marahiel
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