PMID: 230041Dec 1, 1979

Nucleic acid binding and unfolding properties of ribosomal protein S1 and the derivatives S1-F1 and m1-S1

European Journal of Biochemistry
J O ThomasA R Subramanian

Abstract

The nucleic acid binding and unwinding properties of wild-type Escherichia coli ribosomal protein S1 have been compared to those of a mutant form and a large trypsin-resistant fragment, both reported recently [J. Mol. Biol. 127, 41-45 (1979) and J. Biol. Chem. 254, 4309-4312 (1979). The mutant (m1-S1) contains 77% and the fragment (S1-F1) 66% of the polypeptide chain length (approximately 600 amino acid residues) of protein S1. The mutant is active in protein synthesis in vitro; the fragment, although retaining one or more of the functional domains of S1, is inactive in protein synthesis. We find that m1-S1 is is almost as effective as S1 in binding to poly(rU), phage MS2 RNA and simian virus 40 (SV40) DNA, and in unfolding poly(rU) and the helical structures present in MS2 RNA and phi X174 viral DNA. S1-F1, however, binds to poly(rU) and denatured SV40 DNA, but not to MS2 RNA. It unfolds neither poly(rU), nor the residual secondary structure of MS2 RNA or phi X174 viral DNA. Thus, there appears to be a correlation between the loss in ability of S1 to unwind RNA and the loss in its ability to function in protein synthesis.

References

Aug 5, 1978·Journal of Molecular Biology·J O ThomasW Szer
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A KolbW Szer
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D E DraperP H von Hippel
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D G BearH F Noller
Jun 7, 1976·Biochemical and Biophysical Research Communications·W SzerM Boublik
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W SzerS Leffler
Sep 6, 1972·Nature: New Biology·Y GronerM Revel
Jan 15, 1974·Journal of Molecular Biology·G Jay, R Kaempfer
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·W Szer, S Leffler
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J M Hermoso, W Szer
Feb 18, 1970·Biochimica Et Biophysica Acta·M Smolarsky, M Tal
Apr 1, 1966·Journal of Molecular Biology·W Szer

Citations

Oct 4, 2012·Molecular & Cellular Proteomics : MCP·Matthew A LauberJames P Reilly
May 25, 1981·Nucleic Acids Research·A MülschD Riesner
Apr 7, 2005·RNA·Iwona WowerJacek Wower
Nov 19, 2015·Nucleic Acids Research·Heidi GytzCharlotte R Knudsen
Jun 17, 2005·BMC Molecular Biology·Jody BurksJacek Wower
Mar 14, 2007·Biochemical and Biophysical Research Communications·Young Seoub ParkGyoo Yeol Jung
Aug 23, 2005·Biochemical and Biophysical Research Communications·Reinhard DeppingWolfgang Rüger
May 24, 1983·Biochemistry·T Suryanarayana, A R Subramanian
Apr 1, 1984·Archives of Biochemistry and Biophysics·O W OdomB Hardesty
Mar 1, 1982·European Journal of Biochemistry·M KimuraB Wittmann-Liebold

Related Concepts

Bacterial Proteins
ATP-Dependent DNA Helicases
Alkalescens-Dispar Group
Nucleic Acids
Plasma Protein Binding Capacity
Ribosomal Proteins

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.