Nucleic acid detection immunoassay for prostate-specific antigen based on immuno-PCR methodology

Clinical Chemistry
Jonathan E McDermedEleftherios P Diamandis

Abstract

Serum prostate-specific antigen (PSA) concentrations after radical prostatectomy typically become undetectable with the use of current immunometric assay methods. Despite modern surgical techniques, 15%-30% of prostate cancer patients undergoing radical prostatectomy develop a biochemical recurrence during follow-up. Unfortunately, poor analytical sensitivity of standard PSA assays delays biochemical recurrence detection, and because of day-to-day assay imprecision ultrasensitive PSA assays cannot assess PSA kinetics. We developed an immuno-PCR assay for total PSA that has a limit of quantification >10 times lower than current ultrasensitive assays. The 2-site immunometric assay for total PSA employed 2 monoclonal antibodies, one conjugated to a double-stranded DNA label and the other bound to paramagnetic microparticles. After several washing steps, quantification cycles were determined and values were converted to PSA concentrations. We characterized analytical performance and compared accuracy with a commercially available total PSA assay. The limit of quantification was 0.65 ng/L and the assay was linear in the range of 0.25-152.0 ng/L. Total imprecision estimates at PSA concentrations of 3.8, 24.1, and 69.1 ng/L were <15.2...Continue Reading

References

Oct 8, 1987·The New England Journal of Medicine·T A StameyE Redwine
Dec 25, 1993·Nucleic Acids Research·H ZhouT S Papas
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin
Dec 17, 1998·International Journal of Urology : Official Journal of the Japanese Urological Association·Y AraiH Maeda
Jun 14, 2003·The British Journal of Mathematical and Statistical Psychology·Robert A Cribbie, H J Keselman
Mar 20, 2009·The New England Journal of Medicine·Gerald L AndrioleUNKNOWN PLCO Project Team
Mar 20, 2009·The New England Journal of Medicine·Fritz H SchröderUNKNOWN ERSPC Investigators
Oct 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·C Shad ThaxtonChad A Mirkin
Oct 28, 2011·The New England Journal of Medicine·Mary F McNaughton-Collins, Michael J Barry

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Citations

Jul 17, 2014·Prostate Cancer and Prostatic Diseases·J W MoulM A Reynolds
Feb 7, 2016·Molecular and Cellular Probes·Amjad Hayat Khan, Esmaeil Sadroddiny
Jun 29, 2013·Seminars in Oncology·Nicholas G ZaorskyRobert B Den
Jan 3, 2013·Drug Discovery Today·Avisek DeyatiNatalia Novac
Aug 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yinan ChenYingyan Yu
Mar 29, 2016·The Veterinary Quarterly·Anna Luiza F V Assumpção, Rodrigo C da Silva
Feb 14, 2018·Molecular & Cellular Proteomics : MCP·Cyril GalitzineOlga Vitek
Jun 5, 2016·Clinical Chemistry·Natasha Musrap, Eleftherios P Diamandis
Apr 1, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Eleftherios P DiamandisRicardo Azziz
Sep 12, 2018·Diagnostics·Arlou Kristina AngelesHolger Sültmann
Nov 30, 2019·Cancer Letters·Damiano Caputo, Giulio Caracciolo

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