Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood

Nature Nanotechnology
Roya TavallaieJohn Justin Gooding

Abstract

There is intense interest in quantifying the levels of microRNA because of its importance as a blood-borne biomarker. The challenge has been to develop methods that can monitor microRNA expression both over broad concentration ranges and in ultralow amounts directly in a patient's blood. Here, we show that, through electric-field-induced reconfiguration of a network of gold-coated magnetic nanoparticles modified by probe DNA (DNA-Au@MNPs), it is possible to create a highly sensitive sensor for direct analysis of nucleic acids in samples as complex as whole blood. The sensor is the first to be able to detect concentrations of microRNA from 10 aM to 1 nM in unprocessed blood samples. It can distinguish small variations in microRNA concentrations in blood samples of mice with growing tumours. The ultrasensitive and direct detection of microRNA using an electrically reconfigurable DNA-Au@MNPs network makes the reported device a promising tool for cancer diagnostics.

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Citations

Oct 31, 2018·The Analyst·Philip GillespieDanny O'Hare
May 17, 2019·Angewandte Chemie·Xihui GaoChuan Zhang
Nov 14, 2019·Chemical Society Reviews·Mostafa Kamal MasudYusuke Yamauchi
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Sep 21, 2019·Advanced Materials·Lucy GloagJ Justin Gooding
Apr 9, 2020·Frontiers in Chemistry·Rebeca Miranda-CastroNoemí de-Los-Santos-Álvarez
Feb 3, 2019·Analytical and Bioanalytical Chemistry·Roberta D'Agata, Giuseppe Spoto
Jul 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hui WangNongjian Tao
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Apr 23, 2020·Journal of Nanobiotechnology·Congli TangNongyue He
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Apr 27, 2021·Advanced Science·Liangcan HeXiaoyuan Chen

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Methods Mentioned

BETA
biopsies
xenograft
biosensor
PCR
Protein Assay
Assay

Software Mentioned

NanoSight

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