Nucleic acid sequence and affiliation of pLUG10, a novel cadmium resistance plasmid from Staphylococcus lugdunensis

Plasmid
L B ChaouniF Vandenesch

Abstract

Tolerance of Staphylococcus lugdunensis to relatively high levels of cadmium is mediated by a 3117-bp plasmid, pLUG10. Sequencing reveals three major open reading frames (ORFs) in a single orientation. One ORF encompasses the origin of replication and its predicted product (RepL: 350 amino acids (aa)) shows 70% homology in its deduced aa sequence with Rep proteins of the pT181 family. A lagging strand conversion signal (palA) very similar to that of class 1 plasmids is present outside the rep-ori locus. The other two ORFs of 209 and 116 aa show 92.5% homology between their deduced aa sequences and the CadB and CadX peptides from the pOX6 plasmid of Staphylococcus aureus. The CadX-like peptide is 40% homologous to the S. aureus CadC product. Deletion of the C-terminal cadX gene by restriction enzyme digestion or frame-shift inactivation of the cadB gene reduced, but did not completely abolish, cadmium resistance. The two gene products may act cooperatively to confer cadmium resistance in S. lugdunensis.

Citations

Jan 1, 2008·Applied and Environmental Microbiology·Yi-Ywan M ChenRobert A Burne
Aug 9, 2005·Applied and Environmental Microbiology·Naghma NazGeoffrey M Gadd
Jun 25, 1998·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·A MłynarczykJ Jeljaszewicz
Jun 28, 2003·FEMS Microbiology Reviews·Dayle K Blencowe, Andrew P Morby
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Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·S A Khan

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