Nucleic acid sequence-based amplification with oligochromatography for detection of Trypanosoma brucei in clinical samples.

Journal of Clinical Microbiology
Claire M MugasaHenk D F H Schallig

Abstract

Molecular tools, such as real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of human African trypanosomiasis (HAT). Despite good sensitivity, these techniques are not implemented in HAT control programs due to the high cost of the equipment, which is unaffordable for laboratories in developing countries where HAT is endemic. In this study, a simplified technique, oligochromatography (OC), was developed for the detection of amplification products of T. brucei 18S rRNA by NASBA. The T. brucei NASBA-OC test has analytical sensitivities of 1 to 10 parasites/ml on nucleic acids extracted from parasite culture and 10 parasites/ml on spiked blood. The test showed no reaction with nontarget pathogens or with blood from healthy controls. Compared to the composite standard applied in the present study, i.e., parasitological confirmation of a HAT case by direct microscopy or by microscopy after concentration of parasites using either a microhematocrit centrifugation technique or a mini-anion-exchange centrifugation technique, NASBA-OC on blood samples had a sensitivity of 73.0% (95% confidence interval, 60 to 83%), while standard expert...Continue Reading

References

Nov 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·J W Bailey, D H Smith
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jan 1, 1994·Advances in Parasitology·J Pépin, F Milord
Apr 21, 1999·Lancet·M P Barrett
Feb 16, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·D LegrosD Mbulamberi
Jan 4, 2001·Trends in Parasitology·S C WelburnI Maudlin
Jan 5, 2002·Medical Microbiology and Immunology·H D SchalligH Veeken
Mar 6, 2002·Molecular Biotechnology·Birgit DeimanPeter Sillekens
Jul 20, 2002·The Lancet Infectious Diseases·Dominique LegrosPhilippe Büscher
Jan 10, 2003·The American Journal of Tropical Medicine and Hygiene·Magdalena RadwanskaEtienne Pays
Dec 10, 2003·Journal of Clinical Microbiology·Noritaka KubokiIkuo Igarashi
Oct 30, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Benjamin EdvinssonUNKNOWN ECSMID Study Group on Toxoplasmosis
Nov 16, 2004·Diagnostic Microbiology and Infectious Disease·Sven BeckerDietmar Steverding
Jan 18, 2005·Clinical Microbiology Reviews·François ChappuisPhilippe Büscher
Aug 8, 2006·Journal of Clinical Microbiology·S DeborggraeveP Büscher
May 20, 2008·Diagnostic Microbiology and Infectious Disease·Claire M MugasaHenk D F H Schallig
May 27, 2008·Molecular and Biochemical Parasitology·O P AkinwaleT W Fesobi
Sep 26, 2008·The Journal of Infectious Diseases·Stijn DeborggraevePhilippe Büscher

❮ Previous
Next ❯

Citations

Oct 12, 2010·Clinical Microbiology Reviews·J L N BarrattD Stark
Mar 12, 2010·Journal of Clinical Microbiology·Mercedes Pérez-RuizJosé Antonio Carrillo
Jan 19, 2012·PLoS Neglected Tropical Diseases·Claire M MugasaMariska M G Leeflang
Dec 5, 2012·PLoS Neglected Tropical Diseases·Patrick MitashiMarleen Boelaert
Mar 20, 2015·Expert Review of Molecular Diagnostics·Philippe Büscher, Stijn Deborggraeve
Aug 13, 2013·Clinics in Laboratory Medicine·Shawn Vasoo, Bobbi S Pritt
Jan 26, 2016·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Charles D KatoVincent P Alibu
Dec 6, 2011·Diagnostic Microbiology and Infectious Disease·Loreto FuenzalidaVicente Ausina
Jun 11, 2011·Trends in Parasitology·Sally L Wastling, Susan C Welburn
Mar 8, 2011·Trends in Biotechnology·Angelika NiemzDavid S Boyle
Jun 1, 2010·The Lancet Infectious Diseases·Stijn Deborggraeve, Philippe Büscher
May 22, 2010·Tropical Medicine & International Health : TM & IH·Claire M MugasaHenk D F H Schallig
May 26, 2010·Tropical Medicine & International Health : TM & IH·Mamadou CamaraBruno Bucheton
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
May 1, 2012·Journal of Tropical Medicine·Enock MatovuZablon Kithingi Njiru
Sep 14, 2011·Artificial DNA, PNA & XNA·Takehiko ShiraishiPeter E Nielsen
May 3, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jacques KaboréVincent Jamonneau
Feb 1, 2011·FEMS Immunology and Medical Microbiology·Patrice FrancoisJacques Schrenzel
Sep 17, 2014·The Journal of Antimicrobial Chemotherapy·Eniyou C OrieroUmberto D'Alessandro
Aug 20, 2019·Chemical Communications : Chem Comm·Ashley M NewbiggingX Chris Le
Dec 16, 2010·Critical Reviews in Clinical Laboratory Sciences·Fernando Sérgio Rodrigues Ribeiro TelesLuís Joaquim Pina da Fonseca
Nov 17, 2012·Expert Review of Molecular Diagnostics·Stijn Deborggraeve, Philippe Büscher

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.