Nucleic acid testing by public health referral laboratories for public health laboratories using the U.S. HIV diagnostic testing algorithm

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Laura G WesolowskiS Michele Owen

Abstract

Many public health laboratories adopting the U.S. HIV laboratory testing algorithm do not have a nucleic acid test (NAT), which is needed when the third- or fourth-generation HIV screening immunoassay is reactive and the antibody-based supplemental test is non-reactive or indeterminate. Among public health laboratories utilizing public health referral laboratories for NAT conducted as part of the algorithm, we evaluated the percentage of screening immunoassays needing NAT, the number of specimens not meeting APTIMA (NAT) specifications, time to APTIMA result, the proportion of acute infections (i.e., reactive APTIMA) among total infections, and screening immunoassay specificity. From August 2012 to April 2013, 22 laboratories enrolled to receive free APTIMA (NAT) at New York or Florida public health referral laboratories. Data were analyzed for testing conducted until June 2013. Submitting laboratories conducted a median of 4778 screening immunoassays; 0-1.3% (median 0.2%) needed NAT. Of 140 specimens received, 9 (6.4%) did not meet NAT specifications. The median time from specimen collection to reporting the 11 reactive NAT results was ten days, including six days from receipt in the submitting laboratory to shipment to the re...Continue Reading

References

Mar 1, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J S KoopmanK Lange
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Oct 11, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Pollyanna ChavezS Michele Owen
Dec 18, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tammy GoodhueKevin Cranston
Dec 18, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Angela B HutchinsonBernard M Branson

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Citations

Aug 19, 2015·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kevin P DelaneySusan Scheer

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