PMID: 11900837Mar 20, 2002Paper

Nucleo-cytoplasmic localization of influenza virus nucleoprotein depends on cell density and phosphorylation

Virus Research
M BuiG R Whittaker

Abstract

Influenza virus nucleoprotein (NP) plays a major role in the nucleus during virus replication, and is a mediator of viral ribonucleoprotein nuclear import during entry. NP is localized primarily in the nucleus, but can undergo nucleo-cytoplasmic shuttling in heterokaryons (Whittaker et al., 1996a. J. Virol. 70, p. 2743). We have studied NP localization using a stable cell line (3PNP-4) that expresses NP. Intracellular localization of NP was markedly affected by the density of the cell monolayer. It was nuclear in cells grown in sparse culture, but cytoplasmic in dense culture. In phorbol ester-stimulated cells NP was cytoplasmic, but relocalized to the nucleus after treatment with a protein kinase inhibitor. Cell density and phosphorylation-dependent localization of NP appeared to be independent of cell type. Our data suggest that a phosphorylation event is needed either for nuclear export, or to regulate retention of NP in the nucleus, and that regulation may be mediated by kinases activated by cell-cell contact.

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Citations

Oct 28, 2004·Journal of Virology·Ann H ReidJeffery K Taubenberger
Oct 4, 2013·BMC Systems Biology·Yukiko MatsuokaYoshihiro Kawaoka
May 27, 2015·Viruses·Jing LiWenjun Liu
Jun 18, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Leila GangeheiMehran Haidari
Apr 19, 2008·The Journal of General Virology·Sutha SangiambutNopporn Sittisombut
Sep 11, 2009·The Journal of General Virology·Kilian WeigandHeinz Schaller
Mar 7, 2009·The Journal of General Virology·Shohreh MahmoudianManfred Marschall
Jul 28, 2012·Antiviral Chemistry & Chemotherapy·Christopher CianciMark Krystal
Sep 8, 2017·Journal of Virology·Takanari GotoSeiji Hongo
Sep 16, 2005·Journal of Neurovirology·Johan BraskKrister Kristensson
Nov 19, 2005·Journal of Cellular Biochemistry·Maria Pia ValaccoSilvia Moreno

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