Nucleobase-caged phosphoramidites for oligonucleotide synthesis

Current Protocols in Nucleic Acid Chemistry
Alexander Heckel

Abstract

Caged compounds are biologically active agents bearing a photolabile group in a strategic position, which makes them temporarily inactive. These compounds can then be delivered to a biological sample without immediately generating an effect. When the sample is then irradiated, e.g., with a laser in a (confocal) microscope, the activity of the substance is released with exact spatiotemporal and dose control. This unit deals with the synthesis of protected nucleoside phosphoramidites bearing a caging group on the nucleobase, which prevents the nucleobases from forming normal Watson-Crick base pairs. These amidites can be used to generate caged oligodeoxynucleotides with a transitory local perturbation that adds an element of spatiotemporal control to oligonucleotide-based applications.

References

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Dec 30, 2004·Angewandte Chemie·Lenz Kröck, Alexander Heckel
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Sep 24, 2005·Chembiochem : a European Journal of Chemical Biology·Günter MayerAlexander Heckel
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Jul 11, 2006·Angewandte Chemie·Günter Mayer, Alexander Heckel
Sep 20, 2006·Angewandte Chemie·Alexander HeckelGünter Mayer

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Citations

Apr 8, 2016·ACS Chemical Biology·Nicholas B Struntz, Daniel A Harki

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