Nucleocapsid-like particles of dengue-2 virus enhance the immune response against a recombinant protein of dengue-4 virus

Archives of Virology
Laura LazoLisset Hermida

Abstract

In this study, we evaluate in mice a novel formulation containing nucleocapsid-like particles of dengue-2 virus (recNLP) co-immunized with a chimeric protein composed of the dengue-4 envelope domain III fused twice within the meningococcal P64k protein of Neisseria meningitidis (PD24). The animals receiving the PD24-recNLP mixture showed the highest levels of antiviral antibodies. Similar results were obtained for IFNγ secretion levels, indicating a functional Th1 cellular response. Consistently, the percentage of mice surviving after viral challenge was significantly higher for those immunized with the mixture than for those inoculated with PD24 protein alone. In addition, in vivo depletion experiments demonstrated the decisive role of CD4(+) and CD8(+) cells in the protection conferred by immunization with PD24-recNLP. In conclusion, this report demonstrates for the first time the adjuvant capacity of dengue-2 virus recNLP. Additionally, the evidence presented highlights the potential of these particles for enhancing the immune response against heterologous recombinant proteins.

References

Nov 1, 1993·Journal of Virology·H KawanoC J Lai
Dec 1, 1996·Journal of Virology·Y ChenR M Marks
Aug 10, 2000·Scandinavian Journal of Immunology·S GonzálezR Silva
Nov 21, 2000·American Journal of Epidemiology·D W Vaughn
Aug 18, 2001·The American Journal of Tropical Medicine and Hygiene·M SimmonsC G Hayes
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·F TakeshitaD M Klinman
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra RiedlReinhold Schirmbeck
Jun 5, 2003·International Journal for Parasitology·John DonnellyJeffrey B Ulmer
Sep 1, 1958·The American Journal of Tropical Medicine and Hygiene·D H CLARKE, J CASALS
Sep 23, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Carol O TacketMary K Estes
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kelly L WarfieldSina Bavari
Dec 31, 2003·Advances in Virus Research·John T Roehrig
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tazio StorniMartin F Bachmann
Jan 27, 2004·The American Journal of Tropical Medicine and Hygiene·Robert EdelmanWellington Sun
Apr 6, 2004·Immunology and Cell Biology·Mario LobigsEva Lee
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maloy M Mangada, Alan L Rothman
Dec 22, 2005·Biotechnology and Applied Biochemistry·Liz Alvarez-LajonchereSantiago Dueñas-Carrera
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Elizabeth V L Grgacic, David A Anderson
Jul 14, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert Edelman

❮ Previous
Next ❯

Citations

Mar 3, 2012·Applied Microbiology and Biotechnology·Weilong ShangXiancai Rao
Nov 3, 2014·Annual Review of Virology·Theodore C Pierson, Michael S Diamond
Feb 23, 2018·Applied Microbiology and Biotechnology·Hossein FahimiMajid Sadeghizadeh
Sep 30, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lázaro GilLisset Hermida
Jan 25, 2019·Expert Review of Vaccines·Laura LazoLázaro Gil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.