Nucleocytoplasmic transport: a role for nonspecific competition in karyopherin-nucleoporin interactions.

Molecular & Cellular Proteomics : MCP
Jaclyn Tetenbaum-NovattM P Rout

Abstract

Nucleocytoplasmic transport occurs through the nuclear pore complex (NPC), which in yeast is a ~50 MDa complex consisting of ~30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa must be aided across by transport factors, most of which belong to a related family of proteins termed karyopherins (Kaps). These transport factors bind to the disordered phenylalanine-glycine (FG) repeat domains in a family of NPC proteins termed FG nups, and this specific binding allows the transport factors to cross the NPC. However, we still know little in terms of the molecular and kinetic details regarding how this binding translates to selective passage of transport factors across the NPC. Here we show that the specific interactions between Kaps and FG nups are strongly modulated by the presence of a cellular milieu whose proteins appear to act as very weak competitors that nevertheless collectively can reduce Kap/FG nup affinities by several orders of magnitude. Without such modulation, the avidities between Kaps and FG nups measured in vitro are too tight to be compatible with the rapid transport kinetics observed in vivo. We modeled the multivalent interactions between the disorder...Continue Reading

References

Nov 7, 1979·Journal of Theoretical Biology·P H Ehrlich
Mar 1, 1988·The Journal of Cell Biology·S I Dworetzky, C M Feldherr
Jan 1, 1968·Annual Review of Biochemistry·D E Koshland, K E Neet
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S B Zimmerman, A P Minton
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Feb 1, 1993·Analytical Biochemistry·J W Haycock
May 30, 1997·Cell·M P RoutJ D Aitchison
Aug 1, 1997·The Journal of Biological Chemistry·M FloerM Rexach
Feb 23, 2000·The Journal of Cell Biology·M P RoutB T Chait
Mar 17, 2001·The EMBO Journal·K Ribbeck, D Görlich
May 10, 2001·Current Opinion in Cell Biology·E Conti, E Izaurralde
Jun 2, 2001·The Journal of Biological Chemistry·N P AllenM Rexach
Oct 9, 2001·The Journal of Biological Chemistry·R Bussell, D Eliezer
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·I G Macara
Feb 21, 2002·Molecular Biology of the Cell·Nelly Panté, Michael Kann
Feb 28, 2002·The Journal of Biological Chemistry·Daniel GilchristMichael Rexach
Mar 23, 2002·Molecular and Cellular Biology·Deena M LeslieJohn D Aitchison
May 29, 2002·The EMBO Journal·Katharina Ribbeck, Dirk Görlich
Jun 18, 2002·The EMBO Journal·Richard BaylissMurray Stewart
Oct 10, 2002·The Journal of Biological Chemistry·Richard BaylissMurray Stewart
Jan 25, 2003·Molecular & Cellular Proteomics : MCP·Nadia P C AllenMichael Rexach
Feb 13, 2003·Journal of Molecular Biology·Richard P GrantMurray Stewart
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Daniel P DenningMichael Rexach
Mar 27, 2003·Traffic·Janna BednenkoLarry Gerace
Aug 15, 2003·The Journal of Biological Chemistry·Brook Pyhtila, Michael Rexach

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Citations

Jan 9, 2020·The FEBS Journal·Irina L RempelLiesbeth M Veenhoff
May 2, 2014·FEBS Letters·Laure TwyffelsVéronique Kruys
Sep 5, 2015·Nucleus·Roderick Y H LimLarisa E Kapinos
Dec 25, 2012·The Journal of Biological Chemistry·Stephanie RoloffRalph H Kehlenbach
Feb 16, 2016·Nature Structural & Molecular Biology·Jiong MaWeidong Yang
Nov 6, 2015·Molecular Biology of the Cell·Ogheneochukome LolodiShige H Yoshimura
Oct 5, 2016·The Journal of Cell Biology·Benjamin L TimneyMichael P Rout
Oct 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rafael L SchochRoderick Y H Lim
May 20, 2020·Physical Review. E·Paul C Bressloff
Sep 26, 2014·Molecular Biology of the Cell·Mohammad AzimiMohammad R K Mofrad
Nov 28, 2019·Physical Review. E·Laura MaguireLoren E Hough
Feb 7, 2021·International Journal of Biological Macromolecules·Denzelle Lee LyngdohTimir Tripathi
Oct 4, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Michael G OpfermanAnton Zilman
Mar 19, 2021·Communications Biology·Joseph M KelichEmmanuel Skordalakes
Feb 23, 2021·Biophysical Journal·Luke K DavisAnton Zilman
Apr 30, 2021·Journal of Cell Science·Joanna KalitaRoderick Y H Lim
Jul 30, 2021·Frontiers in Cell and Developmental Biology·Yoichi ShinkaiTakamitsu Miyafusa
Jul 30, 2021·Journal of the American Chemical Society·Qi ShenChenxiang Lin
Apr 8, 2014·Chemical Reviews·Monika FuxreiterVladimir N Uversky
Oct 14, 2021·International Journal of Molecular Sciences·Kathleen M LennonTijana Jovanovic-Talisman

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