PMID: 8589453Nov 1, 1995Paper

Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport

Molecular Biology of the Cell
Tokio TaniD L Spector

Abstract

Transport of mRNA from the nucleus to the cytoplasm plays an important role in gene expression in eukaryotic cells. In wild-type Schizosaccharomyces pombe cells poly(A)+ RNA is uniformly distributed throughout the nucleoplasm and cytoplasm. However, we found that a severe heat shock blocks mRNA transport in S. pombe, resulting in the accumulation of bulk poly(A)+ RNA, as well as a specific intron-less transcript, in the nucleoli. Pretreatment of cells with a mild heat shock, which induces heat shock proteins, before a severe heat shock protects the mRNA transport machinery and allows mRNA transport to proceed unimpeded. In heat-shocked S. pombe cells, the nucleolar region condensed into a few compact structures. Interestingly, poly(A)+ RNA accumulated predominantly in the condensed nucleolar regions of the heat-shocked cells. These data suggest that the yeast nucleolus may play a role in mRNA transport in addition to its roles in rRNA synthesis and preribosome assembly.

References

Jun 1, 1978·Cell·A K Hopper, F Banks
Feb 5, 1977·Journal of Molecular Biology·A SpradlingS Penman
Nov 25, 1977·Journal of Molecular Biology·S L McKenzie, M Meselson
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T KadowakiA M Tartakoff
Aug 1, 1992·Seminars in Cell Biology·E Izaurralde, I W Mattaj
Feb 1, 1992·Current Opinion in Cell Biology·R H Singer
Jul 12, 1991·Cell·E A NiggR Lührmann
Dec 1, 1991·Current Opinion in Cell Biology·L E Maquat
Jan 1, 1990·Electron Microscopy Reviews·C M Feldherr, D Akin
Aug 10, 1990·Science·D D Chang, P A Sharp
Mar 1, 1988·The Journal of Cell Biology·S I Dworetzky, C M Feldherr
Jun 1, 1988·Journal of Cellular Physiology·S SadisL A Weber
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T NosakaM Hatanaka
Jun 1, 1989·Experimental Cell Research·H C SmithS M Berget
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Mar 1, 1989·The Journal of Cell Biology·S ZeitlinA Efstratiadis
Jan 1, 1985·Biology of the Cell·R L OchsH Busch
Dec 16, 1988·Science·H J Yost, S Lindquist
Nov 11, 1986·Nucleic Acids Research·T C SantiagoA J Brown

❮ Previous
Next ❯

Citations

Mar 6, 1999·Current Opinion in Microbiology·K A MoranoD J Thiele
Jul 15, 2009·Cloning and Stem Cells·Olga ØstrupPoul Maddox-Hyttel
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
Apr 21, 2005·Cell Structure and Function·Jun-ichi Yoshida, Tokio Tani
Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Shingo Izawa
Sep 7, 2012·PloS One·Ezequiel NázerDaniel O Sánchez
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·K E Sawin, P Nurse
Apr 1, 1997·Current Opinion in Genetics & Development·M S Lee, P A Silver
Aug 15, 1998·Nucleic Acids Research·T Pederson
Apr 7, 2005·Applied and Environmental Microbiology·Shingo IzawaYoshiharu Inoue
Sep 5, 2008·Molecular Biology of the Cell·Hélène Gaillard, Andrés Aguilera
Jun 2, 1997·The Journal of Cell Biology·S HuangD L Spector
Nov 11, 2006·Biochemical and Biophysical Research Communications·Tomoko AndohTokio Tani
Apr 10, 2008·Microscopy Research and Technique·Manuela MalatestaCarlo Zancanaro
Feb 1, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yutaka Haitani, Hiroshi Takagi
Dec 25, 2010·Microscopy Research and Technique·Manuela MalatestaMarco Biggiogera
Feb 18, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sachiko HayashiTokio Tani
Mar 13, 2012·Biochemical and Biophysical Research Communications·Ken'ichi KamoShingo Izawa
Nov 21, 2007·Biochemical and Biophysical Research Communications·Taro MannenTokio Tani
Dec 9, 2014·Nucleus·Pablo Reyes-GutierrezThoru Pederson
Sep 27, 2003·Biochemical and Biophysical Research Communications·Abul Kalam AzadTokio Tani
Jun 28, 1996·Cell·G DreyfussA I Lamond
Jun 24, 2004·The Journal of Biological Chemistry·Shingo IzawaYoshiharu Inoue
Mar 6, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinxin QuanUrsula Stochaj
May 27, 2004·Journal of Cell Science·Takashi IdeueTokio Tani
Mar 1, 1996·Trends in Cell Biology·S Sazer
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·J E McCarthy
Sep 1, 1996·Molecular and Cellular Biology·S LiangA M Tartakoff
May 9, 1998·Science·A I Lamond, W C Earnshaw
Jul 1, 1996·Genes & Development·C SaavedraC N Cole

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.