Nucleolar changes in human phytohaemagglutinin-stimulated lymphocytes

Cell and Tissue Research
F WachtlerH G Schwarzacher

Abstract

The nucleoli of lymphocytes from circulating peripheral blood and from phytohaemagglutinin (PHA)-stimulated cultures (from 2 h-96h) were studied using a silver method, RNA-specific fluorescent staining, and electron microscopy of ultrathin sections. In peripheral blood about 75% of the lymphocytes have one "ring-shaped" nucleolus composed of a distinct fibrillar centre surrounded by a dense pars fibrillaris and little granular material; the remaining lymphocytes showing two or more small "ring-shaped" nucleoli. With PHA stimulation, the number of cells with several nucleoli increases first (from 2 h--12 h). Next, cells containing one or, at most, two large nucleoli with nucleolonema devoid of fibrillar centers are seen (from 4 h on). 34 h after PHA, nucleoli of the "compact" type containing one or more fibrillar centres appear and comprise about 60% of the cells after 72 h. The appearance of more than one nucleolus per cell shortly after PHA administration suggests an activation of additional nucleolar organizer regions (NOR), which fuse to form one or two large nucleoli with nucleolonema. These are then transformed into "compact" nucleoli. The fibrillar centers stasin preferentially with silver. They contain nonchromosomal pro...Continue Reading

Citations

Mar 1, 1987·Cell and Tissue Research·F WachtlerH G Schwarzacher
Jan 1, 1983·Human Genetics·H G Schwarzacher, F Wachtler
Sep 1, 1992·Cell and Tissue Research·A SchedleG Boltz-Nitulescu
Apr 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·O A Sozanskiĭ
May 1, 1984·Experimental Cell Research·K SmetanaH Busch
Jun 1, 1985·Cancer Genetics and Cytogenetics·A DeleenerM Kirsch-Volders
Sep 15, 2006·Nucleic Acids Research·Rebecca A Haeusler, David R Engelke
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M DerenziniV Eusebi
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·K SmetanaH Busch
Nov 1, 1987·Gamete Research·I ChartrainH St-Pierre
Jan 1, 1981·Zentralblatt für Veterinärmedizin. Reihe A·B MayrW Schleger
Oct 1, 1989·Experimental Cell Research·R L Ochs, K Smetana
Jun 1, 1992·Experimental Cell Research·R L Ochs, R I Press
Mar 1, 1991·Experimental Cell Research·T HaafM Schmid

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