Nucleolar localization and mobility analysis of the NF-kappaB repressing factor NRF

Journal of Cell Science
Ina NiedickMario Köster

Abstract

NF-kappaB plays a central role in mediating pathogen and cytokine-stimulated gene transcription. NF-kappaB repressing factor (NRF) has been shown to interact with specific negative regulatory DNA elements (NRE) to mediate transcriptional repression by inhibition of the NF-kappaB activity at certain promoters. mRNA ablation experiments demonstrated that the trans-acting NRF protein is involved in constitutive but not post-stimulated silencing of IFN-beta, IL-8 and iNOS genes by binding to cis-acting NRE elements in their promoters. We have examined the subcellular localization and mobility of the NRF protein. Since neither tagging nor overexpression perturbs NRF localization the GFP-tagged protein was used for detailed localization and mobility studies. Owing to an N-terminal nuclear localization sequence, all NRF fragments that contain this signal show a constitutive nuclear accumulation. C-terminal NRF fragments also localize to the nucleus although no canonical NLS motifs were detected. Full-length NRF is highly enriched in nucleoli and only a small fraction of NRF is found in the nucleoplasm and cytoplasm. This relationship was found to be independent of the protein expression rate. FRAP analysis proved to be a sensitive met...Continue Reading

References

Oct 1, 1972·The Journal of General Virology·H K OieS Baron
Aug 9, 1996·Science·N B ColeJ Lippincott-Schwartz
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Mar 9, 2000·Nature Cell Biology·M A LohrumK H Vousden
Mar 14, 2000·Molecular and Cellular Biology·J D WeberC J Sherr
Mar 25, 2000·Molecular and Cellular Biology·A OumardM Nourbakhsh
Apr 15, 2000·Nature·R D Phair, T Misteli
Jun 15, 2000·Nature Cell Biology·M Carmo-FonsecaI Campos
Dec 26, 2001·Nucleic Acids Research·Laurent FalquetAmos Bairoch
Dec 26, 2001·Nucleic Acids Research·Ivica LetunicPeer Bork
Dec 26, 2001·Nucleic Acids Research·Alex BatemanErik L L Sonnhammer
Dec 26, 2001·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Oct 9, 2002·Genome Research·Lewis Y GeerStephen H Bryant
Nov 14, 2002·Molecular Biology of the Cell·Alexander ScherlJean-Jacques Diaz
Jan 10, 2003·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jul 1, 1993·Trends in Cell Biology·U ScheerG Goessens

❮ Previous
Next ❯

Citations

May 5, 2006·Molecular Biology of the Cell·Ming XuRonald B Emeson
Aug 9, 2011·Journal of Biochemistry·Marc R RebollMahtab Nourbakhsh
Jul 1, 2005·Molecular and Cellular Biology·Lesley A Stark, Malcolm G Dunlop
Dec 16, 2005·Molecular and Cellular Biology·Natali FroeseHansjörg Hauser
Nov 15, 2012·Genes & Cancer·Anand PonnuswamyRobin Fåhraeus
Jan 12, 2012·PLoS Neglected Tropical Diseases·Aldo H TavaresIldinete Silva-Pereira
Feb 21, 2009·EMBO Reports·Edward Emmott, Julian A Hiscox
Sep 3, 2011·Journal of Cellular and Molecular Medicine·Masakazu HashimotoLars O Tjernberg
Nov 30, 2010·The EMBO Journal·Zhongxin LuYong Li
Jan 10, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eun Jung ParkKi Churl Chang
May 16, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·R SchuchtDagmar Wirth
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marta CocciaMaria Gabriella Santoro
Apr 14, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manfred Neumann, Michael Naumann
Feb 6, 2020·The Biochemical Journal·Jana AlexandrovaAnne E Willis
Sep 6, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sascha RotherMahtab Nourbakhsh
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shu-Chuan HoHan-Pin Kuo
Jan 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xin-Jie ChenSai-Juan Chen
Jul 23, 2021·Frontiers in Endocrinology·Sara AndradeMariana P Monteiro
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Allyson M Cochran, Jacki Kornbluth
Dec 11, 2021·Frontiers in Immunology·Yunfei WangChunsheng Kang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.