Abstract
The microtubule binding protein, nucleolar spindle-associated protein 1 (NUSAP1), has a crucial function in mitosis and its expression is closely associated with carcinogenesis. Herein, we aimed to determine the function of NUSAP1 in the development of human esophageal squamous cell carcinoma (ESCC), and the association of NUSAP1 expression with ESCC. Immunohistochemical staining of ESCC tissue sections indicated that NUSAP1 was expressed to a higher degree in tumor tissues than in adjacent nontumor tissues. NUSAP1 levels were relevant closely to histological differentiation (P = 0.049). Overall survival was longer in patients with lower NUSAP1 levels ( P < 0.001). NUSAP1 expression ( P = 0.002), histological differentiation ( P < 0.001), tumor depth ( P = 0.045), lymph node metastases ( P < 0.001), and tumor-node-metastasis staging ( P = 0.008) were greatly associated with overall survival using univariate analysis. Multivariate analysis suggested that histological differentiation ( P = 0.014) and NUSAP1 expression ( P = 0.026) could be independent prognostic markers for ESCC. Additionally, the biological behavior of ESCC cells was investigated in vitro and in vivo. Suppression of NUSAP1 inhibited cellular proliferation and in...Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 10, 2003·The Journal of Cell Biology·Tim RaemaekersGeert Carmeliet
Sep 20, 2005·Molecular Cancer Therapeutics·Demetrius M KokkinakisRussell D Neuner
Mar 3, 2006·Cancer Research·Jen-Liang SuMin-Liang Kuo
Feb 6, 2007·Current Biology : CB·Katharina RibbeckIain W Mattaj
Jul 15, 2009·The Journal of Cell Biology·Shobbir HussainMichaela Frye
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dusan BogunovicNina Bhardwaj
Sep 3, 2010·Journal of Cell Science·An Vanden BoschGeert Carmeliet
Nov 30, 2010·Cellular Signalling·Jyoti IyerMing-Ying Tsai
Jan 5, 2011·Protein & Cell·Jing NieHuizhe Huang
Jun 26, 2013·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·J ChenX Guan
Jul 11, 2013·Bioscience Reports·Nathalia Meireles da CostaLuis Felipe Ribeiro Pinto
Nov 30, 2014·Journal of Cancer Research and Clinical Oncology·Mei YangChangzhi Huang
Jan 15, 2015·The Prostate·Catherine A GordonJames D Brooks
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Mar 11, 2015·Cancer Biology & Therapy·Rong LiuHong-Hao Zhou
Aug 25, 2015·Biological Research·Zhao YangTao Wang
Sep 17, 2015·Gastroenterology·Shinya OhashiManabu Muto
Oct 21, 2015·PloS One·Li ChenZhi-Ming Shao
Nov 12, 2015·PloS One·Atsushi OkamotoKatsuhiro Uzawa
Nov 23, 2015·Molecular Cell·Carmen Dominguez-BrauerTak W Mak
Jul 28, 2016·Oncology Reports·Lu FangSong Wu
Apr 14, 2017·Oncotarget·Catherine A GordonJames D Brooks
Sep 14, 2017·Journal of Experimental & Clinical Cancer Research : CR·Xianqiu WuWei Zhang