Nucleolin Acts as a Scavenger Receptor for Acetylated Low-Density Lipoprotein on Macrophages

Biological & Pharmaceutical Bulletin
Yuichi MikiYasuyuki Fujiwara

Abstract

Although macrophage phagocytoses modified low-density lipoprotein (LDL), excessive accumulation of modified LDL induces macrophage foam cell formation, which is a feature of atherosclerotic plaque. Thus, the identification of scavenger receptor for modified LDL will provide better understanding of an atherosclerotic event. We recently showed that nucleolin expressed on macrophages acts as a scavenger receptor for various endogenous discarded products. Here, we investigated whether or not nucleolin is involved in the uptake of acetylated LDL (AcLDL). In contrast to normal LDL, AcLDL directly bound to immobilized nucleolin. AcLDL exhibited a higher affinity for macrophages than normal LDL. This binding of AcLDL was inhibited by anti-nucleolin antibody and antineoplastic guanine-rich oligonucleotide (AGRO), a nucleolin-specific oligonucleotide aptamer. In addition, AcLDL exhibited a higher affinity for HEK cells transfected with nucleolin than those without. Further, intracellular accumulation of AcLDL was also inhibited by anti-nucleolin antibody. The results of this study suggest that nucleolin expressed on macrophages is a receptor for AcLDL.

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·X ZhaF R Maxfield
Aug 14, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·M LougheedU P Steinbrecher
Jul 16, 2004·Journal of Virology·Santanu BoseAmiya K Banerjee
May 21, 2005·Atherosclerosis·Jane E MurphySreenivasan Ponnambalam
Sep 2, 2005·The Journal of Biological Chemistry·Kazuya HiranoMasatoshi Beppu
Aug 6, 2013·Biological & Pharmaceutical Bulletin·Daisuke OzawaMasatoshi Beppu
Aug 10, 2013·Nature Reviews. Immunology·Johnathan CantonSergio Grinstein
May 13, 2014·Biological & Pharmaceutical Bulletin·Yuichi MikiMasatoshi Beppu
Mar 7, 2015·Biological & Pharmaceutical Bulletin·Yuichi MikiYasuyuki Fujiwara

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