Nucleolin is a receptor for maleylated-bovine serum albumin on macrophages

Biological & Pharmaceutical Bulletin
Yuichi MikiYasuyuki Fujiwara

Abstract

Scavenger receptors have a broad range of functions that include pathogen clearance, and identification of the scavenger receptor family has been of great benefit to the field of physiology. The shuttling-protein nucleolin has recently been shown to possess scavenger receptor-like activity. We therefore investigated whether or not nucleolin is a receptor for maleylated-bovine serum albumin (maleylated-BSA), which is a common ligand for scavenger receptors. Binding and phagocytosis of native control-BSA by thioglycollate-elicited mouse peritoneal macrophages was weak, but that of maleylated-BSA was strong. Surface plasmon-resonance analysis revealed that nucleolin strongly associated with maleylated-BSA but not control-BSA or maleic anhydride. Further, co-treatment of macrophages with anti-nucleolin antibody, but not control-immunoglobulin G, inhibited binding of maleylated-BSA. In addition, antineoplastic guanine rich oligonucleotide (AGRO), a nucleolin-specific oligonucleotide aptamer, inhibited binding of maleylated-BSA. Further, binding of maleylated-BSA to nucleolin-transfected HEK293 cells was higher than that by control HEK cells. These results indicate that nucleolin is a receptor that enables macrophages to recognize ma...Continue Reading

References

May 16, 1998·Biochemical and Biophysical Research Communications·P B AlfordR E Shackelford
Oct 3, 2001·The Journal of Clinical Investigation·V A FadokP M Henson
Nov 26, 2003·Biochimica Et Biophysica Acta·Paola Maderna, Catherine Godson
Dec 11, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Johanna JalkanenSeppo Ylä-Herttuala
Jul 16, 2004·Journal of Virology·Santanu BoseAmiya K Banerjee
Sep 2, 2005·The Journal of Biological Chemistry·Kazuya HiranoMasatoshi Beppu
Sep 15, 2007·Biochemical and Biophysical Research Communications·Yuichi MikiMasatoshi Beppu
Jan 6, 2010·Journal of Cancer Research and Clinical Oncology·Tatsuro WatanabeMasami Suganuma
Aug 13, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Hong FengXiang-An Li
Apr 3, 2012·Biological & Pharmaceutical Bulletin·Emiri OguriMasatoshi Beppu
Jan 1, 1972·Methods in Enzymology·P J Butler, B S Hartley
Aug 6, 2013·Biological & Pharmaceutical Bulletin·Daisuke OzawaMasatoshi Beppu
Aug 10, 2013·Nature Reviews. Immunology·Johnathan CantonSergio Grinstein
May 13, 2014·Biological & Pharmaceutical Bulletin·Yuichi MikiMasatoshi Beppu

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Sep 4, 2015·Biological & Pharmaceutical Bulletin·Yuichi MikiYasuyuki Fujiwara

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