Nucleolin regulates 14-3-3ζ mRNA and promotes cofilin phosphorylation to induce tunneling nanotube formation.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Sunayana DagarSivaram V S Mylavarapu

Abstract

Tunneling nanotubes (TNTs) mediate intercellular communication between animal cells in health and disease, but the mechanisms of their biogenesis and function are poorly understood. Here we report that the RNA-binding protein (RBP) nucleolin, which interacts with the known TNT-inducing protein MSec, is essential for TNT formation in mammalian cells. Nucleolin, through its RNA-binding domains (RBDs), binds to and maintains the cytosolic levels of 14-3-3ζ mRNA, and is, therefore, required for TNT formation. A specific region of the 3'-untranslated region (UTR) of the 14-3-3ζ mRNA is likely to be involved in its regulation by nucleolin. Functional complementation experiments suggest that nucleolin and 14-3-3ζ form a linear signaling axis that promotes the phosphorylation and inactivation of the F-actin depolymerization factor cofilin to induce TNT formation. MSec also similarly inactivates cofilin, but potentiates TNT formation independent of the nucleolin-14-3-3ζ axis, despite biochemically interacting with both proteins. We show that 14-3-3ζ and nucleolin are required for the formation of TNTs between primary mouse neurons and astrocytes and in multiple other mammalian cell types. We also report that the Caenorhabditis elegans o...Continue Reading

References

May 1, 1974·Genetics·S Brenner
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·L MaM W Kirschner
Dec 12, 2001·Nature Cell Biology·Serge MoskalenkoMichael A White
Oct 4, 2002·Current Biology : CB·Antje Gohla, Gary M Bokoch
Dec 18, 2003·The Journal of Biological Chemistry·Tapas K SenguptaEleanor K Spicer
May 27, 2004·The Journal of Biological Chemistry·Sarah E M MeekHelen Piwnica-Worms
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karnail SinghLori R Covey
Mar 2, 2006·Molecular and Cellular Biology·Yong JiangJ Eric Russell
May 31, 2007·Journal of Cell Science·Nan MaKiichi Fukui
Feb 19, 2008·Cancer Research·Jack ZhangG Tim Bowden
Feb 10, 2009·Nature Cell Biology·Karine GoussetChiara Zurzolo
Apr 14, 2009·Cancer Immunology, Immunotherapy : CII·Dorota Hoja-ŁukowiczAnna Lityńska
Aug 7, 2009·Molecular Pharmacology·Sridharan SoundararajanDaniel J Fernandes
Aug 8, 2009·The Journal of Biological Chemistry·Kerry L InderJohn F Hancock
Aug 18, 2010·Communicative & Integrative Biology·Hiroshi OhnoShunsuke Kimura
Sep 21, 2011·Biochimica Et Biophysica Acta·Xiang Wang, Hans-Hermann Gerdes
Mar 9, 2012·Journal of Cell Science·Saïda Abounit, Chiara Zurzolo
Dec 12, 2012·Cell Death & Disease·X SunY Zhang
Dec 12, 2012·Cell and Tissue Research·Shunsuke KimuraHiroshi Ohno
Dec 19, 2012·Mechanisms of Development·Hans-Hermann GerdesXiang Wang
Sep 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aaron BoudreauMina J Bissell
Apr 30, 2014·Communicative & Integrative Biology·Magnus Wiger AustefjordXiang Wang
Jan 13, 2015·Cell Death and Differentiation·X Wang, H-H Gerdes
Jul 15, 2015·European Journal of Cell Biology·Sajjad Sisakhtnezhad, Leila Khosravi
Apr 7, 2016·Journal of Proteome Research·Anna SalvettiAnna Greco
Jun 27, 2017·Developmental Biology·Lara M LindenDavid R Sherwood
Oct 19, 2017·International Journal of Molecular Sciences·Daniel Brandwein, Zhixiang Wang
Dec 7, 2017·Experimental Cell Research·A D'AloiaM Ceriani

❮ Previous
Next ❯

Citations

Aug 18, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Aysha DilnaSangeeta Nath
Nov 24, 2021·The Biochemical Journal·Sunayana DagarSivaram V S Mylavarapu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.