Nucleomorphin. A novel, acidic, nuclear calmodulin-binding protein from dictyostelium that regulates nuclear number

The Journal of Biological Chemistry
Michael A Myre, Danton H O'Day

Abstract

Probing of Dictyostelium discoideum cell extracts after SDS-PAGE using (35)S-recombinant calmodulin (CaM) as a probe has revealed approximately three-dozen Ca(2+)-dependent calmodulin binding proteins. Here, we report the molecular cloning, expression, and subcellular localization of a gene encoding a novel calmodulin-binding protein (CaMBP); we have called nucleomorphin, from D. discoideum. A lambdaZAP cDNA expression library of cells from multicellular development was screened using a recombinant calmodulin probe ((35)S-VU1-CaM). The open reading frame of 1119 nucleotides encodes a polypeptide of 340 amino acids with a calculated molecular mass of 38.7 kDa and is constitutively expressed throughout the Dictyostelium life cycle. Nucleomorphin contains a highly acidic glutamic/aspartic acid inverted repeat (DEED) with significant similarity to the conserved nucleoplasmin domain and a putative transmembrane domain in the carboxyl-terminal region. Southern blotting reveals that nucleomorphin exists as a single copy gene. Using gel overlay assays and CaM-agarose we show that bacterially expressed nucleomorphin binds to bovine CaM in a Ca(2+)-dependent manner. Amino-terminal fusion to the green fluorescence protein (GFP) showed tha...Continue Reading

References

Feb 1, 1992·Current Opinion in Cell Biology·R E Cheney, M S Mooseker
Jan 1, 1990·Developmental Genetics·T DingermannK Nerke
Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 11, 1990·Nucleic Acids Research·G Maridor, E A Nigg
Jan 1, 1989·Journal of Cell Science. Supplement·C DingwallS M Dilworth
Jan 1, 1988·Preparative Biochemistry·D L NewtonC B Klee
Oct 1, 1987·The Journal of Cell Biology·W C Earnshaw
May 1, 1982·Molecular and Cellular Biology·W F LoomisJ A Schmidt
Apr 6, 1995·Biochemical and Biophysical Research Communications·T RatajczakR F Minchin
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
May 30, 1994·Biochemical and Biophysical Research Communications·M A LydanD H O'Day
Mar 22, 1996·The Journal of Biological Chemistry·J T AndersonH W Jarrett
Sep 1, 1996·Differentiation; Research in Biological Diversity·F Horn, J Gross
Feb 7, 1997·The Journal of Biological Chemistry·X Ye, R D Sloboda
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A R Rhoads, F Friedberg
Jun 20, 1997·The Journal of Biological Chemistry·S B LudbrookM Strom
May 1, 1997·Research in Microbiology·S HellsternR Mutzel
Jun 22, 1999·The Journal of Biological Chemistry·P O TsvetkovA A Makarov
Aug 25, 2000·The Journal of Biological Chemistry·F SafadiA S Reddy

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Citations

Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Mar 6, 2003·Cellular Signalling·Danton H O'Day
Mar 25, 2005·Molecular Biology of the Cell·Catherine P ChiaLaura K Smoyer
Sep 21, 2011·Biological Reviews of the Cambridge Philosophical Society·Danton H O'Day, Alex Keszei
Aug 15, 2008·Computational Biology and Chemistry·Fernanda BareaDiego Bonatto
Sep 28, 2007·Cellular Signalling·Andrew Catalano, Danton H O'Day
Aug 25, 2004·Biochemical and Biophysical Research Communications·Michael A Myre, Danton H O'Day
Mar 13, 2014·Biological Reviews of the Cambridge Philosophical Society·Danton H O'Day, Aldona Budniak
Nov 13, 2004·Biochimica Et Biophysica Acta·Michael A Myre, Danton H O'Day
Jul 14, 2004·Biochemical and Biophysical Research Communications·Danton H O'Day, Michael A Myre
May 11, 2005·Biochemical and Biophysical Research Communications·Danton H O'DayMichael A Myre
Nov 6, 2013·Biochimica Et Biophysica Acta·Martin W Berchtold, Antonio Villalobo
Sep 15, 2004·Biochimica Et Biophysica Acta·Michael A Myre, Danton H O'Day
Dec 20, 2014·Biochemical and Biophysical Research Communications·Andrew Catalano, Danton H O'Day
Jun 1, 2006·Functional Plant Biology : FPB·Qiusheng ZhangYingguo Zhu
Nov 28, 2019·Biological Reviews of the Cambridge Philosophical Society·Danton H O'DayRobert J Huber

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