PMID: 2507249Jan 1, 1989Paper

Nucleoplasmic and cytoplasmic glycoproteins

Ciba Foundation Symposium
G W HartW G Kelly

Abstract

We have described a new form of protein glycosylation in which N-acetylglucosamine is glycosidically linked to the hydroxyl of serine or threonine (O-GlcNAc). Unlike most other forms of protein glycosylation, O-GlcNAc is predominantly localized in the nuclear and cytoplasmic compartments of cells, where it occurs on important nuclear pore glycoproteins, well-characterized cytoskeletal proteins, as well as on many chromatin proteins, including factors that regulate gene transcription. Gas-phase protein sequencing of three O-GlcNAc-modified proteins has identified a common structural feature at sites of O-GlcNAc addition. An assay for UDP-GlcNAc:polypeptide O-GlcNAc transferase has been developed. The enzyme appears to be membrane-associated, its active site is cytoplasmic, and it has an absolute requirement for Mn2+. We are now purifying this glycosyltransferase, characterizing its substrate specificity, and determining the extent of elongation of attached saccharide moieties. The functions of O-GlcNAc remain largely unknown, but it may be important in blocking phosphorylation sites, it may be required for the assembly of specific multiprotein complexes, it might serve as a nuclear transport signal, or it may be directly involve...Continue Reading

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