Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis

Cell Division
HartonoRichard W Wong

Abstract

Nuclear pore complexes (NPCs) act as nano-turnstiles within nuclear membranes between the cytoplasm and nucleus of mammalian cells. NPC proteins, called nucleoporins (Nups), mediate trafficking of proteins and RNA into and out of the nucleus, and are involved in a variety of mitotic processes. We previously reported that Nup62 localizes to the centrosome and mitotic spindle during mitosis, and plays a role in centrosome homeostasis. However, whether Nup58, a Nup62 subcomplex protein, also localizes to spindle poles is unknown. Herein, we show that Nup58 localizes to the nuclear rim during interphase, and to mitotic spindles, centrosomes, and midbodies during mitosis. Our confocal microscopy, live-cell imaging, and stimulated emission depletion nanoscopy results also demonstrated that Nup58 localized to the centrosomes during metaphase and relocalized to midbodies during abscission. Depletion of Nup58 resulted in centrosomal abnormalities and delayed abscission. Nup58 localized at the centrosomes and mitotic spindle during metaphase and relocalized at midbodies during abscission. This study highlights the important role of Nup58 in mitosis.

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Citations

Dec 19, 2020·Biochemical Society Transactions·Nike HeinßEdward A Lemke
Aug 28, 2021·Cells·Richard W Wong

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Methods Mentioned

BETA
GTPase
confocal microscopy
immunoprecipitation
pull down
transfection
PCR

Software Mentioned

CellSens
Adobe Photoshop
Prism
ImageJ
GraphPad
Huygens Professional Deconvolution

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