PMID: 6170612Sep 1, 1981Paper

Nucleoprotein cytochemistry during oogenesis in a unisexual fish, Poecilia formosa

The Histochemical Journal
P J MonacoJ S Balsano

Abstract

Cytochemical methods and electron microscopy were used to study changes in the chemical composition of nuclear, nucleolar and perinuclear bodies during the early stages of oocyte development in Poecilia formosa, an apomictic species of fish that produces only female offspring. Prominent accumulations of ribonucleoprotein (RNP) occur in nucleoli and appear on either side of the nuclear envelope during diplotene. In certain planes of section, RNP material seems to be in transit across this interface. En bloc acid extractions or RNAase treatment abolished basophilia and markedly reduced the electron density of both nucleoli and cytoplasmic nucleolar-like bodies. DNA-specific fluorescent probes such as mithramycin failed to reveal nucleolar cores in poeciliid oocytes, although the same procedures showed unequivocal localization of GC-rich DNA cores within multiple nucleoli of diplotene oocytes from Xenopus laevis or the rainbow trout, Salmo gairdneri. Also, cytological hybridization studies, utilizing [3H]rRNA as a probe for nucleolar DNA, failed to show localizations of silver grains above nucleoli in autoradiograms of poeciliid oocytes. Feulgen-stained pachytene oocytes of P. formosa have twice the number of chromosome strands se...Continue Reading

References

May 1, 1975·Stain Technology·F H Kasten, R Lala
Feb 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Johannisson, B Thorell
Sep 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P B Coulson, R Tyndall
Apr 2, 1976·Cell and Tissue Research·M Vlad
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A Lima-De-FariaT Gustafsson
May 1, 1972·Biological Reviews of the Cambridge Philosophical Society·H C Macgregor
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J G Gall, M L Pardue
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J G Gall
Dec 1, 1969·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·L M Prehn, E M Rasch
Nov 1, 1953·Stain Technology·A D CONGER, L M FAIRCHILD
Jan 1, 1955·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE
Nov 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G G GLENNER, R D LILLIE
Sep 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A D DEITCH
May 1, 1962·Stain Technology·R J TOLBERT
Mar 6, 1964·Science·H C MACGREGOR, T M UZZELL
Feb 24, 1965·Zeitschrift für Zellforschung und mikroskopische Anatomie·S S GURAYA
Sep 1, 1980·Evolution; International Journal of Organic Evolution·Bruce J TurnerRobert R Miller

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