Nucleoprotein gel electrophoresis for the analysis of nucleosomes and their positioning and mobility on DNA

Methods : a Companion to Methods in Enzymology
S Pennings

Abstract

Nucleoprotein gel electrophoresis, as well as its use in chromatin research, is reviewed from its early application in the characterization of native nucleosome composition to current uses in analyzing transcription factor-nucleosome complexes and in visualizing multiple nucleosome positioning. Despite our incomplete understanding of the principles behind the separation of particles in native polyacrylamide, gels, powerful new applications of the method have emerged in recent years. This offers the potential for novel experimental strategies, such as those that have led to the discovery of nucleosome mobility.

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Citations

Feb 25, 2014·Nucleic Acids Research·Justin A NorthJennifer J Ottesen
Feb 6, 2007·Biophysical Journal·Martin Castelnovo, Sébastian Grauwin
Jun 26, 2007·Journal of Molecular Biology·Alfonso G Fernandez, John N Anderson
Jun 12, 2008·Systems Biology in Reproductive Medicine·Igor B NazarovAndrei O Zalensky

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