PMID: 8970956Dec 1, 1996Paper

Nucleoprotein viral RNA and mRNA of Thogoto virus: a novel "cap-stealing" mechanism in tick-borne orthomyxoviruses?

Journal of Virology
F WeberG Kochs

Abstract

Tick-borne Thogoto virus (THOV) represents the prototype virus of a new genus in the Orthomyxoviridae family. Its genome consists of six segments of negative-sense, single-stranded RNA. We have cloned and sequenced the fifth genomic segment, which codes for the viral nucleoprotein (NP). The deduced amino acid sequence shows 43% similarity to the NP of Dhori virus, a related tick-transmitted orthomyxovirus, and about 14% sequence similarity to those of the influenza viruses. To reveal the mechanism by which THOV initiates mRNA synthesis, we characterized the 5' ends of the NP mRNAs. Transcripts were recognized by a cap-specific monoclonal antibody, indicating that THOV mRNAs are capped. Surprisingly, no large heterogeneous extensions were found at the 5' end, as would have been expected if THOV were using a classical "cap-stealing" mechanism. We therefore propose that THOV is stealing only the cap structure with one or two additional nucleotides from cellular mRNAs to generate appropriate primers for initiation of viral mRNA transcription.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Aug 1, 1991·Trends in Genetics : TIG·R A Lamb, C M Horvath
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1990·Journal of Virology·D Garcin, D Kolakofsky
Jan 1, 1990·Methods in Enzymology·J Hein
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Oct 3, 1970·Nature·R Rott, C Scholtissek
Jun 25, 1980·Nucleic Acids Research·A J Caton, J S Robertson
Sep 1, 1983·Journal of Virology·D R LondoD P Nayak
May 1, 1983·Virology·J P ClerxD H Bishop
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I UlmanenR M Krug

❮ Previous
Next ❯

Citations

Feb 6, 1999·The Journal of Biological Chemistry·G Kochs, O Haller
Nov 22, 2000·The Journal of General Virology·E WagnerG Kochs
Sep 5, 2001·Journal of Virology·E WagnerG Kochs
Dec 1, 2009·Indian Journal of Microbiology·Ragnhild ToennessenEspen Rimstad
Oct 24, 2000·Journal of Virology·G KochsO Haller
Jan 29, 2003·Journal of Virology·Kathrin HagmaierGeorg Kochs
Mar 4, 2000·The Journal of Biological Chemistry·M TrostO Haller
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G Kochs, O Haller
Dec 6, 2011·Nature Reviews. Microbiology·Etienne DecrolyBruno Canard
Jun 22, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Espen Rimstad, Siri Mjaaland
Oct 14, 2011·The Journal of General Virology·Ana Valeria BussettiW Ian Lipkin
Jun 20, 2018·Scientific Reports·Melbourne Rio TalactacTetsuya Tanaka
May 17, 2019·Journal of Virology·Jan SpitaelsXavier Saelens
Oct 5, 2019·Nucleic Acids Research·Rhian JonesJuan Reguera
Sep 15, 1998·Methods : a Companion to Methods in Enzymology·G KochsO Haller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.