PMID: 7016181Apr 28, 1981Paper

Nucleoside 5'-[gamma-S]triphosphates will initiate transcription in isolated yeast nuclei

Biochemistry
G J Ide

Abstract

Transcription was carried out in isolated nuclei by endogenous ribonucleic acid (RNA) polymerase in the presence of nucleoside 5'-[gamma-S]triphosphates. The resulting 5'-gamma-thiophosphate on the synthesized RNA allows separation of in vitro initiated RNA from bulk RNA. Analysis of this in vitro initiated RNA shows that 5S RNA and pre-tRNA are initiated in vitro by RNA polymerase III. Yeast RNA polymerase III also reinitiates discrete distribution of RNA species as large as 28 S. The RNA populations initiated with adenosine 5'-[gamma-S]triphosphate and guanosine 5'-[gamma-S]triphosphate are different.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R S ZitomerB D Hall
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J E HopperL B Rowe
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jun 18, 1974·Biochemical and Biophysical Research Communications·D Gratecos, E H Fischer
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·N M Maizels
Oct 1, 1972·FEBS Letters·J Hindley, S M Page
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey

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Citations

Mar 1, 1993·Plant Molecular Biology·P Schweizer, K Hahlbrock
Nov 1, 1981·Current Genetics·G J Ide, C A Saunders
Jan 1, 1981·The International Journal of Biochemistry·S P GregoryM J Pocklington
Jan 1, 1984·The International Journal of Biochemistry·D P HuangJ F Chiu
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I Y Sun, V G Allfrey
Sep 11, 2004·Biochemical and Biophysical Research Communications·Francesca MagheriniAlessandra Modesti
May 1, 1986·Molecular and Cellular Biology·J F Jerome, J A Jaehning

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