PMID: 3772380Dec 1, 1986Paper

Nucleoside transporter of cerebral microvessels and choroid plexus

Journal of Neurochemistry
R N Kalaria, S I Harik

Abstract

The nucleoside transporter of cerebral microvessels and choroid plexus was identified and characterized using [3H]nitrobenzylthioinosine (NBMPR) as a specific probe. [3H]NBMPR bound reversibly and with high affinity to a single specific site in particulate fractions of cerebral microvessels, choroid plexus, and cerebral cortex of the rat and the pig. The dissociation constants (KD 0.1-0.7 nM) were similar in the various tissue preparations from each species, but the maximal binding capacities (Bmax) were about fivefold higher in cerebral microvessels and choroid plexus than in the cerebral cortex. Nitrobenzylthioguanosine and dipyridamole were the most potent competitors for [3H]NBMPR binding. Several naturally occurring nucleosides displaced specific [3H]NBMPR binding to cerebral microvessels in vitro, in a rank order that correlated well with their ability to cross the blood-brain barrier in vivo. Adenosine analogues and theophylline were less effective in displacing [3H]NBMPR binding than in displacing adenosine receptor ligands. Photoactivation of cerebral microvessels and choroid plexus bound with [3H]NBMPR followed by solubilization and polyacrylamide gel electrophoresis labeled a protein(s) with a molecular weight of app...Continue Reading

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Citations

Feb 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R N Kalaria, S I Harik
Mar 15, 2006·BioTechniques·Pengfei SongCharles R Yates
Jan 1, 1988·Annals of Neurology·R O Brady, R R O'Neill
Feb 16, 1999·Journal of Neurotrauma·J M GiddayT S Park
Jun 17, 2020·Neuroscience Bulletin·Bruno G Frenguelli, Nicholas Dale
Nov 17, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Xianmin ZengWilliam J Freed
Jul 1, 1994·The American Journal of Physiology·L SobrevíaD L Yudilevich
Jun 6, 2000·Advanced Drug Delivery Reviews·X Li, W K Chan
Apr 25, 1988·Biochimica Et Biophysica Acta·M TorresM T Miras-Portugal

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