PMID: 4894329Feb 1, 1969Paper

Nucleoside triphosphate pools and the regulation of RNA synthesis in E. coli

Proceedings of the National Academy of Sciences of the United States of America
G Edlin, G S Stent

Abstract

Amino acid starvation of "stringent RNA control" (RC(str)) strains of Escherichia coli (E. coli) results in the cessation of net protein and net RNA synthesis, whereas "relaxed RNA control" (RC(rel)) strains continue net RNA synthesis under the same conditions of amino acid starvation. This report tests further the hypothesis that net RNA synthesis is markedly reduced during amino acid starvation of RC(str) strains as a result of reduction in the supply of substrates of the RNA polymerase. Bacterial ribonucleoside triphosphate pool levels were measured before and after the arrest of protein synthesis in RC(str) and RC(rel) strains. Protein synthesis was inhibited either by addition of trimethoprim to the medium or by the use of a mutant having a temperature-sensitive valyl-tRNA synthetase. The ribonucleoside triphosphate pool levels do not decline significantly during inhibition of protein synthesis in RC(str) strains under either condition, and there is no apparent correlation between the measured pool levels and the residual rate of net RNA synthesis in RC(str) and RC(rel) strains. Thus, these data argue against the hypothesis that the regulation of RNA synthesis is mediated by the availability of substrates of the RNA polyme...Continue Reading

References

Oct 10, 1968·Biochemical and Biophysical Research Communications·A S Bagnara, L R Finch
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D P Nierlich
Nov 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A Y ShihP Lengyel
Mar 14, 1967·Journal of Molecular Biology·G Edlin, J Neuhard
May 14, 1967·Journal of Molecular Biology·J Gallant, M Cashel
Dec 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·G S STENT, S BRENNER
Jun 1, 1964·Journal of Molecular Biology·K MARCKER, F SANGER

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Citations

Sep 8, 1975·Molecular & General Genetics : MGG·A MutoS Osawa
Jan 1, 1975·Molecular & General Genetics : MGG·A Muto
Jan 1, 1975·Advances in Enzyme Regulation·R J Harvey, I K Dev
Jun 1, 1973·European Journal of Biochemistry·R Kaplan, N Silman
Nov 5, 2003·Journal of Bacteriology·Casper Møller JørgensenJan Martinussen
Jan 21, 1970·Biochimica Et Biophysica Acta·Y SokawaY Kaziro
May 31, 1979·Annals of the New York Academy of Sciences·R D Hotchkiss
Mar 10, 2009·Studies in History and Philosophy of Biological and Biomedical Sciences·Hans-Jörg Rheinberger
Dec 30, 1972·Journal of Molecular Biology·P Donini
May 5, 1973·Journal of Molecular Biology·A Cozzone, P Donini
May 5, 1973·Journal of Molecular Biology·A J Cozzone, G S Stent
Jan 2, 1975·Biochemical and Biophysical Research Communications·Y CenatiempoA J Cozzone
Jul 26, 2017·ELife·Barak A Cohen
Jun 1, 1971·Journal of Bacteriology·W RuffD A Goldthwait
Apr 1, 1970·Journal of Bacteriology·P P Dennis, R K Herman
Oct 1, 1971·Journal of Bacteriology·L Huzyk, D J Clark
Sep 1, 1970·Bacteriological Reviews·G A O'Donovan, J Neuhard

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