Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison

Histochemistry and Cell Biology
Jéril DegrouardJacques-Edmond Fléchon

Abstract

The nucleoskeleton is a complex structure involved in structural and functional organisation of the genome in eukaryotic cells. As little information on the nucleoskeleton is available from early embryonic development stages, we describe here the morphology and composition of the nucleoskeleton in cleaving bovine embryos (stages 1-16 cells). Ultrastructural observations were performed using thick resinless sections after chromatin removal by nucleases. The localisation of nucleoskeleton-related lamins A and C, NuMA, SRm160 and hnRNP H was tested by immunofluorescence. The characteristic structures of the nucleoskeleton (nuclear lamina, core filaments and the 'diffuse' nucleoskeleton) were present throughout all embryonic stages studied, although less discernable during the 1-cell stage. Lamins A and C as well as the NuMA protein were observed in embryo nuclei from the 1-cell stage; a diffuse hnRNP H and speckled SRm160 immunofluorescence appeared from the 4-cell stage. During the 8- to 16-cell stages (major transcriptional activation), the immunofluorescence patterns were identical with those of differentiated cells (fibroblasts). The temporal pattern of immunolabelling confirmed that transcription and splicing compartmentalisa...Continue Reading

Citations

Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Nov 12, 2005·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Jan 5, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Helen A FosterJoanna M Bridger
Jul 30, 2011·Journal of Biosciences·Parul Varma, Rakesh K Mishra
Sep 14, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Heide Schatten, Qing-Yuan Sun
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Yunping DaiLin Liu
Sep 15, 2005·Molecular Reproduction and Development·Vanessa J HallAndrew J French
Jul 2, 2010·Reproduction, Fertility, and Development·Richard D W KellyKeith H S Campbell

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