Nucleosomal structure of active and inactive c-myc genes.

The Journal of Biological Chemistry
A PullnerD Eick

Abstract

The nucleosomal structure of active and inactive c-myc genes has been analyzed in detail in undifferentiated and differentiated cells of the promyelocytic leukemia cell line HL60. The c-myc P2 promoter was never found in nucleosomal configuration, no matter whether c-myc was expressed or not. Differences in the nucleosomal structure, however, were found in the promoter upstream region proximal to a previously described DNase I-hypersensitive site I, at the P0 promoter, and at the P1 promoter and upstream thereof. In these regions nucleosomes were detected in differentiated but not undifferentiated HL60 cells. Similar patterns of nucleosomes as found for active and inactive c-myc genes in HL60 cells were found for active and inactive episomal c-myc genes in stably transfected B cell lines. In these cell lines three activation stages could be described for episomal c-myc constructs: (i) uninducible, (ii) inducible, and (iii) induced. Significant differences in the nucleosomal structure of c-myc were observed for the uninducible and inducible stages, but not for the inducible and induced stages.

References

Jan 1, 1992·Annual Review of Biochemistry·K B MarcuA J Patel
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A BossoneK B Marcu
Nov 5, 1991·Journal of Molecular Biology·S Kumar, M Leffak
Jan 1, 1991·Advances in Cancer Research·C A Spencer, M Groudine
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M P DuyaoG E Sonenshein
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S W HiebertJ R Nevins
Jun 29, 1989·Nature·E KakkisK Calame
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Apr 1, 1995·Current Opinion in Genetics & Development·D L Bentley
Apr 21, 1995·The Journal of Biological Chemistry·E F MichelottiD Levens
Jan 11, 1995·Nucleic Acids Research·J MautnerA Polack
Jan 1, 1994·Annual Review of Biochemistry·S M ParanjapeJ T Kadonaga
Apr 8, 1994·Cell·A P Wolffe
Jun 1, 1994·Molecular and Cellular Biology·D Dufort, A Nepveu
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Brys, N Maizels
Dec 1, 1993·Molecular and Cellular Biology·K J RiggsK Calame
Jan 1, 1996·Advances in Cancer Research·M Henriksson, B Lüscher

❮ Previous
Next ❯

Citations

Jul 25, 2003·Experimental Cell Research·Scott W BlumePeter D Emanuel
Aug 8, 2001·Leukemia Research·L P ZhangY J He
Jan 28, 1998·Leukemia Research·D Kaul, M Kaur
Jun 10, 1998·The EMBO Journal·M A Martinez-BalbásT Kouzarides
Sep 30, 2008·Nucleic Acids Research·Hillel I SimsGavin R Schnitzler
Nov 20, 2001·Molecular and Cellular Biology·I CollinsD Levens
Oct 29, 2004·Molecular and Cellular Biology·Alison A Hill, Paul R Riley
May 23, 2009·BMC Cancer·Laura ArceMarian L Waterman
Nov 10, 2000·Biochemical and Biophysical Research Communications·T SimonssonM Vorlickova
Jul 16, 2015·Cancer Biology & Therapy·Christos VaklavasScott W Blume
Mar 30, 2001·The Journal of Biological Chemistry·T AlbertD Eick

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.