Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin

Genes & Development
Jinsook SonDanny Reinberg

Abstract

Polycomb-repressive complex 2 (PRC2) comprises specific members of the Polycomb group of epigenetic modulators. PRC2 catalyzes methylation of histone H3 at Lys 27 (H3K27me3) through its Enhancer of zeste (Ezh) constituent, of which there are two mammalian homologs: Ezh1 and Ezh2. Several ancillary factors, including Jarid2, modulate PRC2 function, with Jarid2 facilitating its recruitment to target genes. Jarid2, like Ezh2, is present in poorly differentiated and actively dividing cells, while Ezh1 associates with PRC2 in all cells, including resting cells. We found that Jarid2 exhibits nucleosome-binding activity that contributes to PRC2 stimulation. Moreover, such nucleosome-binding activity is exhibited by PRC2 comprising Ezh1 (PRC2-Ezh1), in contrast to PRC2-Ezh2. The presence of Ezh1 helps to maintain PRC2 occupancy on its target genes in myoblasts where Jarid2 is not expressed. Our findings allow us to propose a model in which PRC2-Ezh2 is important for the de novo establishment of H3K27me3 in dividing cells, whereas PRC2-Ezh1 is required for its maintenance in resting cells.

References

Aug 10, 1999·Seminars in Cell & Developmental Biology·J J Jacobs, M van Lohuizen
May 11, 2000·Methods in Molecular Biology·K LugerT J Richmond
May 16, 2000·Circulation Research·Y LeeG E Lyons
Jun 8, 2001·Molecular and Cellular Biology·D O'CarrollT Jenuwein
Aug 2, 2003·The Journal of Biological Chemistry·Tae-Gyun KimYoungsook Lee
Jun 4, 2004·Molecular Cell·Liangjun WangRichard S Jones
Dec 8, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jooyoung JungYoungsook Lee
May 27, 2005·Current Biology : CB·Nathan D MontgomeryTerry Magnuson
May 23, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Takashi TakeuchiShunzo Kondo
Feb 27, 2007·Cell·Bernd SchuettengruberGiacomo Cavalli
Mar 7, 2007·Molecular and Cellular Biology·Diego PasiniKristian Helin
Nov 26, 2008·Molecular Cell·Raphael MargueronDanny Reinberg
Mar 19, 2009·Nucleic Acids Research·Hana KimJoomyeong Kim
Sep 22, 2009·Nature·Raphael MargueronSteven J Gamblin
Feb 4, 2010·Genes & Development·Gang LiDanny Reinberg
Dec 21, 2010·PLoS Genetics·Eric M MendenhallBradley E Bernstein
Dec 22, 2010·Molecular Cell·Jing ZhaoJeannie T Lee
Jan 21, 2011·Nature·Raphaël Margueron, Danny Reinberg
May 10, 2011·Molecular Cell·Frank W SchmitgesNicolas H Thomä

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Citations

Jul 6, 2014·Nucleic Acids Research·Chen DavidovichThomas R Cech
Aug 12, 2015·Biochimica Et Biophysica Acta·Minghui YueYuya Ogawa
Aug 16, 2014·Immunological Reviews·Rhys S Allan, Stephen L Nutt
Mar 24, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Payal Jain, Luciano Di Croce
May 13, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Kristen M Meldi, Maria E Figueroa
Apr 14, 2016·Seminars in Cell & Developmental Biology·Stefan F Pinter
Oct 2, 2014·The FEBS Journal·Pedro VizánLuciano Di Croce
Jun 3, 2014·Molecular Cell·Catherine Cifuentes-RojasJeannie T Lee
Jan 27, 2015·Annual Review of Plant Biology·Iva Mozgova, Lars Hennig
Jul 26, 2014·Cell Reports·Yuri B Schwartz, Vincenzo Pirrotta
Nov 5, 2014·Cell and Tissue Research·Matthew Corley, Kristen L Kroll
May 21, 2016·Trends in Cell Biology·Marianne EntrevanGiacomo Cavalli
Jun 4, 2016·Science·Andrea Piunti, Ali Shilatifard
Mar 28, 2017·Nature Structural & Molecular Biology·Beatrice BodegaValerio Orlando
May 20, 2014·Nature Structural & Molecular Biology·Reinhard KalbJürg Müller
Jan 31, 2018·Nature Structural & Molecular Biology·Simon PoepselEva Nogales
May 12, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer A JacobsenBarbara L Kee
Oct 24, 2017·Nature Structural & Molecular Biology·Xueyin WangThomas R Cech
Dec 15, 2015·Science Advances·Sergi ArandaLuciano Di Croce
Oct 24, 2018·Protein Science : a Publication of the Protein Society·M Daniel RickettsRonen Marmorstein
Feb 23, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vincent LoubiereGiacomo Cavalli
May 28, 2019·Genes & Development·Jia-Ray YuDanny Reinberg
Dec 24, 2018·The EMBO Journal·Diaa Al-RaawiAditi Kanhere
Jun 13, 2019·Acta Pharmacologica Sinica·Yi ShiH Eric Xu
Jan 20, 2018·Science·Vignesh KasinathEva Nogales
Aug 28, 2019·Nature Communications·Roberta RagazziniRaphaël Margueron

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